Fluorescence probe enhanced spectrofluorimetric method for the determination of sparfloxacin in tablets and biological fluids.

Yttrium-sensitized fluorescence was used to develop a sensitive and simple spectrofluorimetric method for the determination of sparfloxacin. The method is based on the strong fluorescence of sparfloxacin after adding the fluorescence probe yttrium in buffer solution (pH = 8), and various factors influencing fluorescence were investigated. Under optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of sparfloxacin over the range 8 × 10(-7) to 1.4 × 10(-5)  mol L(-1) with a correlation coefficient of 0.9997. The detection limit (S/N = 3) was determined as 9.01 × 10(-8)  mol L(-1). The mechanism of the sensitizing effect of probe was discussed. This method has been successfully applied for the determination of sparfloxacin in pharmaceuticals, human urine and serum samples; the result obtained was satisfactory.