Tg2576 小鼠海马中细胞凋亡相关蛋白 caspase-3 和 NF-κB 的表达。
Expression of the apoptosis-related proteins caspase-3 and NF-kappaB in the hippocampus of Tg2576 mice.
机构信息
Institute of Neurobiology, Nursing College of Henan University, Kaifeng 475004, China.
出版信息
Neurosci Bull. 2010 Feb;26(1):37-46. doi: 10.1007/s12264-010-6122-3.
OBJECTIVE
To investigate the relations between neuroapoptosis and the onset and development of Alzheimer's disease (AD), especially the role of NF-kappaB in the regulation of neuroapoptosis.
METHODS
Caspase-3 and NF-kappaB (p50) expressions in the CA3 region of the hippocampus in APPswe Tg2576 transgenic mice were studied from postnatal day 0-180, using Nissl staining, immunohistochemistry and RT-PCR methods.
RESULTS
Both neuronal apoptosis and NF-kappaB activity decreased gradually with the increase of age in wild type and Tg2576 mice. However, the number of caspase-3-positive or NF-kappaB-positive pyramidal cells in Tg2576 mice was greater than that in age-matched wild type mice, with significant differences after postnatal day 14 (P < 0.01 or P < 0.05). Linear regression analyses of caspase-3 and NF-kappaB expression demonstrated a correlation between neuroapoptosis and activity of NF-kappaB.
CONCLUSION
The process of neuroapoptosis is consistent with the onset and development of AD. Furthermore, the observed correlation between neuroapoptosis and NF-kappaB activity suggests a role of NF-kappaB in hippocampal neuroapoptosis.
目的
研究神经细胞凋亡与阿尔茨海默病（AD）发病和发展的关系，特别是 NF-κB 在神经细胞凋亡调控中的作用。
方法
采用 Nissl 染色、免疫组织化学和 RT-PCR 方法，研究 APPsweTg2576 转基因小鼠出生后 0-180 天海马 CA3 区 caspase-3 和 NF-κB（p50）的表达。
结果
野生型和 Tg2576 小鼠的神经细胞凋亡和 NF-κB 活性均随年龄的增加而逐渐下降，但 Tg2576 小鼠的 caspase-3 阳性或 NF-κB 阳性锥体细胞数多于同龄野生型小鼠，差异有统计学意义（P<0.01 或 P<0.05）。Caspase-3 和 NF-κB 表达的线性回归分析表明，神经细胞凋亡与 NF-κB 活性之间存在相关性。
结论
神经细胞凋亡的过程与 AD 的发病和发展一致。此外，观察到的神经细胞凋亡与 NF-κB 活性之间的相关性表明 NF-κB 在海马神经细胞凋亡中起作用。
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