在中国，急性呼吸道感染患儿中人类偏肺病毒的流行情况及临床和分子特征。

Prevalence and clinical and molecular characterization of human metapneumovirus in children with acute respiratory infection in China.

机构信息

Department of Pediatrics, The People's Hospital, Hunan Province, China.

出版信息

Pediatr Infect Dis J. 2010 Feb;29(2):131-4. doi: 10.1097/inf.0b013e3181b56009.

DOI:10.1097/inf.0b013e3181b56009

PMID:20135829

Abstract

BACKGROUND

Human metapneumovirus (HMPV), a newly discovered paramyxovirus, has been associated with acute respiratory tract infections (ARTIs). However, the prevalence and molecular characteristics of HMPV in China are still unclear.

METHODS

A total of 661 nasopharyngeal aspirates (NPA) specimens were collected from 661 children with ARTIs between December 2006 and November 2008. Specimens were screened for HMPV by reverse transcription-polymerase reaction. All positive amplification products were confirmed by sequencing.

RESULTS

HMPV was detected in 45 patients (6.80%) of the 661 children. The HMPV-infected patients were from 29 days to 9 years of age. A high incidence of HMPV infection (84.4%) was observed during the winter-spring season. Of the 45 HMPV-positive patients, 25 (55.6%) were co-infected with other respiratory viruses, and respiratory syncytial virus (RSV) was the most common additional respiratory virus. The most common clinical diagnosis was bronchopneumonia (57.8%) and cough (88.9%) was the most common clinical symptom. Phylogenetic analysis of the F gene revealed that 80% of the HMPV detected were A2, 2.2% were A1, and 17.8% were B1. Statistical analyses showed that sex, ages, seasons, and severity of the disease did not correlate with HMPV genotype (P = 0.986, 0.347, 0.660, 0.252), but viral coinfection with HMPV increased hospitalization rates (P = 0.005).

CONCLUSIONS

HMPV was frequently detected in the pediatric patients with ARTI in China. RSV was the most common coinfection virus and coinfection increased hospitalization rates. All HMPV subgroups except B2 cocirculated and there was no association found between HMPV genotypes and severity of disease.

摘要

背景

人偏肺病毒（HMPV）是一种新发现的副黏病毒，与急性呼吸道感染（ARTIs）有关。然而，HMPV在中国的流行情况和分子特征尚不清楚。

方法

2006 年 12 月至 2008 年 11 月，共采集 661 例急性呼吸道感染患儿的 661 份鼻咽抽吸物（NPA）标本，采用反转录-聚合酶链反应（RT-PCR）方法检测 HMPV。所有阳性扩增产物均经测序证实。

结果

在 661 例患儿中，45 例（6.80%）检测到 HMPV。HMPV 感染患儿的年龄为 29 天至 9 岁，高发季节为冬春季（84.4%）。45 例 HMPV 阳性患儿中，25 例（55.6%）合并其他呼吸道病毒感染，其中最常见的是呼吸道合胞病毒（RSV）。最常见的临床诊断为支气管肺炎（57.8%），最常见的临床症状是咳嗽（88.9%）。F 基因的系统进化分析显示，80%的 HMPV 为 A2 型，2.2%为 A1 型，17.8%为 B1 型。统计分析显示，性别、年龄、季节和疾病严重程度与 HMPV 基因型无相关性（P = 0.986，0.347，0.660，0.252），但 HMPV 合并感染增加了住院率（P = 0.005）。