Lake Rob, King Nicola, Sexton Kerry, Bridgewater Philippa, Campbell Donald
ESR Christchurch Science Centre, PO Box 29-181, Christchurch, New Zealand.
N Z Med J. 2009 Dec 11;122(1307):48-54.
To describe and quantify laboratory testing of faecal samples for enteric pathogens as a component of the reporting pyramid of acute gastrointestinal illness (AGI) in New Zealand.
Postal survey of community and hospital laboratories throughout New Zealand conducted in mid-2006, requesting data from the 2005 calendar year.
Of the 47 laboratories eligible for the survey, responses were received from 35 (74%, 16 hospital laboratories, 12 community laboratories, five hospital and community laboratories, and two Public Health Laboratories). Based on survey data and extrapolation it was estimated that approximately 250,000 faecal samples were received by New Zealand laboratories in 2005. The majority of these (77%) were requested by primary healthcare providers on people in the community. Routine testing of these samples would include bacteria (Salmonella, Shigella, Campylobacter, Yersinia) and parasites (Cryptosporidium, Giardia) and (depending principally on the age of the patient) rotavirus. Testing for other pathogens was comparatively infrequent. The frequency of detection of a pathogen in community samples was estimated as approximately 20%.
The positivity rate of 20% for faecal samples from people in the community is consistent with overseas results. Although there was considerable variation in the testing methods employed by the laboratories the methods were considered appropriate based on consultation with ESR Public Health and Reference Laboratory staff. These data on the number and type of samples, and positivity rate , will assist in the determination of a reporting pyramid for AGI in New Zealand.
描述并量化对粪便样本进行肠道病原体实验室检测的情况,作为新西兰急性胃肠疾病(AGI)报告金字塔的一个组成部分。
2006年年中对新西兰各地的社区和医院实验室进行邮政调查,要求提供2005年日历年的数据。
在47家符合调查条件的实验室中,有35家(74%)回复了调查,其中包括16家医院实验室、12家社区实验室、5家医院和社区联合实验室以及2家公共卫生实验室。根据调查数据和推断,估计2005年新西兰实验室共收到约25万个粪便样本。其中大部分(77%)是由初级医疗保健提供者针对社区人群提出检测要求的。这些样本的常规检测项目包括细菌(沙门氏菌、志贺氏菌、弯曲杆菌、耶尔森氏菌)和寄生虫(隐孢子虫、贾第虫),以及(主要取决于患者年龄)轮状病毒。对其他病原体的检测相对较少。社区样本中病原体的检测频率估计约为20%。
社区人群粪便样本20%的阳性率与海外结果一致。尽管各实验室采用的检测方法存在很大差异,但经与ESR公共卫生和参考实验室工作人员协商,这些方法被认为是合适的。这些关于样本数量和类型以及阳性率的数据,将有助于确定新西兰AGI的报告金字塔。