冠状病毒基因组 RNA 5'近端序列的组特异性结构特征。
Group-specific structural features of the 5'-proximal sequences of coronavirus genomic RNAs.
机构信息
Leiden Institute of Chemistry, Department of Molecular Genetics, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.
出版信息
Virology. 2010 May 25;401(1):29-41. doi: 10.1016/j.virol.2010.02.007. Epub 2010 Mar 4.
Global predictions of the secondary structure of coronavirus (CoV) 5' untranslated regions and adjacent coding sequences revealed the presence of conserved structural elements. Stem loops (SL) 1, 2, 4, and 5 were predicted in all CoVs, while the core leader transcription-regulating sequence (L-TRS) forms SL3 in only some CoVs. SL5 in group I and II CoVs, with the exception of group IIa CoVs, is characterized by the presence of a large sequence insertion capable of forming hairpins with the conserved 5'-UUYCGU-3' loop sequence. Structure probing confirmed the existence of these hairpins in the group I Human coronavirus-229E and the group II Severe acute respiratory syndrome coronavirus (SARS-CoV). In general, the pattern of the 5' cis-acting elements is highly related to the lineage of CoVs, including features of the conserved hairpins in SL5. The function of these conserved hairpins as a putative packaging signal is discussed.
对冠状病毒（CoV）5'非翻译区和相邻编码序列的二级结构的全球预测显示出保守结构元件的存在。所有的 CoV 都预测到茎环（SL）1、2、4 和 5，而核心启动子转录调节序列（L-TRS）仅在一些 CoV 中形成 SL3。I 组和 II 组 CoV 的 SL5 具有一个大的序列插入，能够与保守的 5'-UUYCGU-3'环序列形成发夹，而 IIa 组 CoV 除外。结构探测证实了 I 组人冠状病毒-229E 和 II 组严重急性呼吸综合征冠状病毒（SARS-CoV）中这些发夹的存在。总的来说，5'顺式作用元件的模式与 CoV 的谱系高度相关，包括 SL5 中保守发夹的特征。讨论了这些保守发夹作为潜在包装信号的功能。
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