USDA-ARS, Southeast Poultry Research Laboratory, 934 College Station Rd., Athens, GA 30605, USA.
J Clin Microbiol. 2010 May;48(5):1892-4. doi: 10.1128/JCM.00148-10. Epub 2010 Mar 17.
Eight Newcastle disease virus isolates from Pakistan were sequenced and characterized. A PCR matrix gene assay, designed to detect all avian paramyxovirus 1, did not detect four of the isolates. A new matrix gene test that detected all isolates was developed. Phylogenetic analysis and pathotyping confirmed that virulent viruses of different genotypes are circulating in Pakistan.
从巴基斯坦分离到 8 株新城疫病毒并进行了测序和鉴定。一种设计用于检测所有禽副黏病毒 1 型的 PCR 基质基因检测方法未能检测到其中的 4 株。现已开发出一种新的基质基因检测方法,可检测所有分离株。系统进化分析和致病型鉴定证实,不同基因型的强毒病毒正在巴基斯坦流行。