Peng Yue-lin, Yang Min-na, Cai Xiao-bu
Tibet Agricultural and Animal Husbandry College, Linzhi, China.
Ying Yong Sheng Tai Xue Bao. 2010 May;21(5):1258-63.
This study was based on the isolation and identification of arbuscular mycorrhizal (AM) fungi collected from the alpine Stipa steppe of north Tibet, and focused on the influence of soil texture, pH, organic matter, and available P on the spore density, isolation frequency, relative abundance, importance value, species diversity, and species evenness of the AM fungi. In the rhizosphere soil of the steppe, a total of 15 species AM fungi were isolated and identified, including 9 species of Glomus, 6 species of Acaulospora, and 1 species of Scutellospora. Among them, Glomus and Acaulospora were the dominant genera, and Glomus claroideum and Acaulospora laevis were the dominant species. In the soils with different texture, the spore density, isolation frequency, relative abundance, and importance value of the AM fungi all showed a trend of Glomus > Acaulospora > Scutellospora. Soil pH value had no significant effects on the species composition of AM fungi. However, the isolation frequency, relative abundance, and importance value of Glomus and Acaulospora showed an increasing trend with increasing soil pH, while Scutellospora showed the contrary trend. In the soils with different organic matter content, the spore density of AM fungi all showed a trend of Glomus > Acaulospora > Scutellospora, while the distribution of AM fungi had no definite pattern. The species richness and spore density of AM fungi were less affected by the soil available P content, but the species diversity and evenness showed an increasing trend with the increasing content of soil available P.
本研究基于从藏北高寒针茅草原采集的丛枝菌根(AM)真菌的分离与鉴定,重点关注土壤质地、pH值、有机质和有效磷对AM真菌孢子密度、分离频率、相对丰度、重要值、物种多样性和物种均匀度的影响。在该草原的根际土壤中,共分离鉴定出15种AM真菌,其中球囊霉属9种、无梗囊霉属6种、盾巨孢囊霉属1种。其中,球囊霉属和无梗囊霉属为优势属,明球囊霉和光壁无梗囊霉为优势种。在不同质地的土壤中,AM真菌的孢子密度、分离频率、相对丰度和重要值均表现为球囊霉属>无梗囊霉属>盾巨孢囊霉属的趋势。土壤pH值对AM真菌的物种组成无显著影响。然而,球囊霉属和无梗囊霉属的分离频率、相对丰度和重要值随土壤pH值升高呈增加趋势,而盾巨孢囊霉属则呈相反趋势。在不同有机质含量的土壤中,AM真菌的孢子密度均表现为球囊霉属>无梗囊霉属>盾巨孢囊霉属的趋势,而AM真菌的分布无明确规律。AM真菌的物种丰富度和孢子密度受土壤有效磷含量影响较小,但物种多样性和均匀度随土壤有效磷含量的增加呈增加趋势。