[在俄罗斯、乌克兰、哈萨克斯坦和吉尔吉斯斯坦分离出的新城疫病毒强毒株的分子遗传特征]
[Molecular genetic characteristics of the newcastle disease virus velogenic strains isolated in Russia, Ukraine, Kazakhstan, and Kirghizia].
作者信息
Korotetskiĭ I S, Bogoiavlenskiĭ A P, Prilipov A G, Usachev E V, Usacheva O V, Turgambetova A S, Zaĭtseva I A, Kydyrmanov A, Shakhvorostova L I, Saiatov M Kh, Borisov V V, Pchelkina I P, Gerilovich A P, Berezin V E
出版信息
Vopr Virusol. 2010 Jul-Aug;55(4):29-32.
The F gene fragment of 79 Newcastle disease virus (NDV) strains isolated from domestic and synanthropic birds in Kazakhstan, Kirghizia, Ukraine, and Russia in 1993 to 2007 was comparatively analyzed. Phylogenetic analysis of test isolates and reference NDV strains obtained from the GenBank was carried out by polymerase chain reaction with subsequent sequencing and comparative analysis of 154-bp nucleotide sequences in the main functional region of the F gene. All newly characterized isolates belong to three NDV genotype VII subgroups: VIIa, VIIb, VIId. The results show it necessary to monitor of NDV strains isolated in the CIS countries since the spread of NDV among migratory and synanthropic birds (pigeons, crows, and jackdaws) poses a serious threat to commercial poultry industry.
对1993年至2007年期间从哈萨克斯坦、吉尔吉斯斯坦、乌克兰和俄罗斯的家禽及伴人鸟类中分离出的79株新城疫病毒（NDV）的F基因片段进行了比较分析。通过聚合酶链反应对测试分离株和从GenBank获得的参考NDV毒株进行系统发育分析，随后对F基因主要功能区的154个碱基对核苷酸序列进行测序和比较分析。所有新鉴定的分离株属于新城疫病毒基因型VII的三个亚组：VIIa、VIIb、VIId。结果表明，鉴于新城疫病毒在候鸟和伴人鸟类（鸽子、乌鸦和寒鸦）中的传播对商业家禽业构成严重威胁，有必要对独联体国家分离出的新城疫病毒毒株进行监测。
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