羟基红花黄色素A对油酸和脂多糖诱导的大鼠急性肺损伤的保护作用

[Protective effect of hydroxysafflor yellow A against acute lung injury induced by oleic acid and lipopolysaccharide in rats].

作者信息

Wang Xiao-fei, Jin Ming, Tong Jing, Wu Wei, Li Jin-rong, Zang Bao-xia

机构信息

Department of Pharmacology, Beijing Anzhen Hospital, Capital Medical University-Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.

出版信息

Yao Xue Xue Bao. 2010 Jul;45(7):940-4.

PMID:20931796

Abstract

This study is to investigate the pharmacological effect and mechanism of action of hydroxysafflor yellow A (HSYA) on acute lung injury (ALI). The rat ALI was induced by oleic acid and lipopolysaccharide (LPS) injection. The incidence of acidosis, PaO2 (arterial blood oxygen pressure), W/D (wet weight/dry weight) and lung index (LI) were measured. Electron microscope and optical microscope were applied to observe lung morphological changes in rat. RT-PCR was used to determine TNF-alpha and ICAM-1 mRNA level. Inhibition effect of HSYA on plasma inflammatory cytokine expression was measured by ELISA. HSYA could alleviate pulmonary edema, reduce acidosis, keep PaO2 from descending, inhibit inflammatory cell infiltration, inhibit rat lung TNF-alpha and ICAM-1 mRNA expression and plasma IL-6 and IL-1beta level elevation. HSYA is an effective ingredient to remit ALI induced by oleic acid and LPS in rat.

摘要

本研究旨在探讨羟基红花黄色素A（HSYA）对急性肺损伤（ALI）的药理作用及作用机制。通过注射油酸和脂多糖（LPS）诱导大鼠ALI。检测酸中毒发生率、动脉血氧分压（PaO2）、湿重/干重（W/D）及肺指数（LI）。应用电子显微镜和光学显微镜观察大鼠肺组织形态学变化。采用逆转录聚合酶链反应（RT-PCR）检测肿瘤坏死因子-α（TNF-α）和细胞间黏附分子-1（ICAM-1）mRNA水平。采用酶联免疫吸附测定（ELISA）检测HSYA对血浆炎症细胞因子表达的抑制作用。HSYA可减轻肺水肿，减轻酸中毒，防止PaO2下降，抑制炎症细胞浸润，抑制大鼠肺组织TNF-α和ICAM-1 mRNA表达及血浆白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）水平升高。HSYA是缓解大鼠油酸和LPS诱导的ALI的有效成分。