用脉冲电子电子双共振光谱研究生物分子复合物。
Studying biomolecular complexes with pulsed electron-electron double resonance spectroscopy.
机构信息
Biomedical Sciences Research Complex, Centre of Magnetic Resonance, University of St Andrews, St Andrews KY16 9ST, UK.
出版信息
Biochem Soc Trans. 2011 Jan;39(1):128-39. doi: 10.1042/BST0390128.
The function of biomolecules is intrinsically linked to their structure and the complexes they form during function. Techniques for the determination of structures and dynamics of these nanometre assemblies are therefore important for an understanding on the molecular level. PELDOR (pulsed electron-electron double resonance) is a pulsed EPR method that can be used to reliably and precisely measure distances in the range 1.5-8 nm, to unravel orientations and to determine the number of monomers in complexes. In conjunction with site-directed spin labelling, it can be applied to biomolecules of all sizes in aqueous solutions or membranes. PELDOR is therefore complementary to the methods of X-ray crystallography, NMR and FRET (fluorescence resonance energy transfer) and is becoming a powerful method for structural determination of biomolecules. In the present review, the methods of PELDOR are discussed and examples where PELDOR has been used to obtain structural information on biomolecules are summarized.
生物分子的功能与其结构以及它们在功能过程中形成的复合物内在相关。因此，用于确定这些纳米级组装体的结构和动力学的技术对于在分子水平上的理解非常重要。PELDOR（脉冲电子-电子双共振）是一种脉冲 EPR 方法，可用于可靠且精确地测量 1.5-8nm 范围内的距离，以揭示取向并确定复合物中的单体数量。与定点自旋标记结合使用，它可以应用于水溶液或膜中的各种大小的生物分子。因此，PELDOR 是 X 射线晶体学、NMR 和 FRET（荧光共振能量转移）方法的补充，并且正在成为生物分子结构测定的有力方法。在本综述中，讨论了 PELDOR 的方法，并总结了 PELDOR 用于获得生物分子结构信息的示例。
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