男性年龄对不育人群精液质量、精子 DNA 碎片化和染色体异常的影响。
The effects of male aging on semen quality, sperm DNA fragmentation and chromosomal abnormalities in an infertile population.
机构信息
Department of Cytogenetic and Reproductive Biology, Farhat Hached, University Teaching Hospital, 4000 Sousse, Tunisia.
出版信息
J Assist Reprod Genet. 2011 May;28(5):425-32. doi: 10.1007/s10815-011-9537-5. Epub 2011 Feb 3.
PURPOSE
To investigate the effects of male aging on semen quality, DNA fragmentation and chromosomal abnormalities in the spermatozoa of infertile patients and fertile men.
METHODS
Semen samples of 140 infertile patients (24-76 years) and 50 men with proven fertility (25-65 years) were analyzed according to WHO guidelines. DNA fragmentation was detected by TUNEL assay, while aneuploidy was assessed by FISH.
RESULTS
In the patient group, semen volume and vitality of spermatozoa decreased significantly with age, while sperm concentration showed a statistically significant increase with age. DNA fragmentation as well as disomy of sex chromosomes and disomy 8 did not show a statistically significant change with age. However, the diploidy rate was significantly increased with patient's age. In the control group, conventional semen parameters as well as DNA fragmentation and chromosomal abnormalities did not show a statistically significant with age.
CONCLUSION
Increased age in infertile men is associated with an increase in sperm concentration and diploidy, as well as a decline in semen volume and sperm vitality. However motility, morphology and DNA fragmentation are not affected by male age.
目的
研究男性年龄对不育患者和生育能力正常男性精子精液质量、DNA 碎片和染色体异常的影响。
方法
根据世界卫生组织（WHO）的指南，对 140 例不育患者（24-76 岁）和 50 例生育能力正常男性（25-65 岁）的精液样本进行分析。采用 TUNEL 法检测 DNA 碎片，用荧光原位杂交法（FISH）评估非整倍体。
结果
在患者组中，精子体积和活力随年龄增长显著下降，而精子浓度随年龄增长呈统计学意义上的显著增加。DNA 碎片以及性染色体和 8 号染色体三体均未随年龄发生统计学显著变化。然而，二倍体率随患者年龄的增长显著增加。在对照组中，常规精液参数以及 DNA 碎片和染色体异常均与年龄无统计学显著相关性。
结论
不育男性年龄的增加与精子浓度和二倍体率的增加以及精液量和精子活力的下降有关。然而，精子的运动能力、形态和 DNA 碎片不受男性年龄的影响。
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