Isolation, culture, and characterization of human umbilical cord stroma-derived mesenchymal stem cells.

As the collection and isolation of human bone marrow-derived mesenchymal stem cells (MSCs) require invasive and often undesirable procurement procedures, investigators have begun to seek alternative sources of human MSC including the umbilical cord stroma. Here we describe the noninvasive isolation, culture, and basic characterization of human umbilical cord stroma-derived mesenchymal stem cells (hUCS-MSCs). Although some technical and observational variations exist between laboratories, there has been a relatively common consensus regarding the immunological and functional characteristics of hUCS-MSCs. Successful in vitro and in vivo differentiation to several lineages makes these cells an invaluable stem cell source, deserving further testing as a cellular therapy or other applications in regenerative medicine. Therefore, the isolation and culture of hUCS-MSCs still need better clarification to ultimately build an optimal standard procedure among laboratories, tissue banks, and clinics.