基于纳米多孔金和石墨烯的用于检测人血清绒毛膜促性腺激素的无标记电流型免疫传感器。
Label-free amperometric immunosensor for the detection of human serum chorionic gonadotropin based on nanoporous gold and graphene.
机构信息
Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, People's Republic of China.
出版信息
Anal Biochem. 2011 Jul 15;414(2):196-201. doi: 10.1016/j.ab.2011.03.019. Epub 2011 Mar 22.
A label-free amperometric immunosensor for fast and sensitive assay of human serum chorionic gonadotropin (hCG) is presented. hCG was immobilized on nanoporous gold (NPG) foils and using hydroquinone (HQ) redox species as indicator. The variation of amperometric response to the concentration of hCG, the target antigen, was evaluated by cyclic voltammetry in phosphate-buffered solution. Taking advantage of dual-amplification effects of the NPG foils and graphene sheets (GSs), the immunosensor exhibited a specific response to hCG in the range of 0.5-40.00 ng ml(-1) with a detection limit of 0.034 ng ml(-1) under optimal conditions. It was demonstrated that our proposed method possesses good accuracy, acceptable precision, and reproducibility. The NPG showed a better sensitizing effect and stability as immobilization matrices.
一种无标记的安培免疫传感器，用于快速灵敏地测定人血清绒毛膜促性腺激素（hCG）。hCG 固定在纳米多孔金（NPG）箔上，并使用对苯二酚（HQ）氧化还原物种作为指示剂。在磷酸盐缓冲溶液中通过循环伏安法评估了对 hCG（目标抗原）浓度的电流响应变化。利用 NPG 箔和石墨烯片（GS）的双重放大效应，该免疫传感器在 0.5-40.00ngml（-1）范围内对 hCG 表现出特异性响应，在最佳条件下检测限为 0.034ngml（-1）。结果表明，所提出的方法具有良好的准确性、可接受的精密度和重现性。NPG 作为固定基质具有更好的敏化效果和稳定性。
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