使用平衡木评估小鼠的运动平衡和协调能力。
Assessment of motor balance and coordination in mice using the balance beam.
作者信息
Luong Tinh N, Carlisle Holly J, Southwell Amber, Patterson Paul H
机构信息
Department of Biology, California Institute of Technology, USA.
出版信息
J Vis Exp. 2011 Mar 10(49):2376. doi: 10.3791/2376.
Abstract
Brain injury, genetic manipulations, and pharmacological treatments can result in alterations of motor skills in mice. Fine motor coordination and balance can be assessed by the beam walking assay. The goal of this test is for the mouse to stay upright and walk across an elevated narrow beam to a safe platform. This test takes place over 3 consecutive days: 2 days of training and 1 day of testing. Performance on the beam is quantified by measuring the time it takes for the mouse to traverse the beam and the number of paw slips that occur in the process. Here we report the protocol used in our laboratory, and representative results from a cohort of C57BL/6 mice. This task is particularly useful for detecting subtle deficits in motor skills and balance that may not be detected by other motor tests, such as the Rotarod.
摘要
脑损伤、基因操作和药物治疗可导致小鼠运动技能的改变。精细运动协调和平衡可通过横梁行走试验进行评估。该测试的目的是让小鼠保持直立并走过一个高架的狭窄横梁到达一个安全平台。此测试连续进行3天：2天训练和1天测试。通过测量小鼠穿过横梁所需的时间以及在此过程中发生的爪滑倒次数来量化在横梁上的表现。在这里，我们报告我们实验室使用的方案，以及一组C57BL/6小鼠的代表性结果。这项任务对于检测其他运动测试（如转棒试验）可能无法检测到的运动技能和平衡方面的细微缺陷特别有用。
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