端粒重复序列扩增法：检测端粒酶活性

Telomeric repeat amplification protocol: measuring the activity of the telomerase.

作者信息

Xin Huawei

机构信息

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

出版信息

Methods Mol Biol. 2011;735:107-11. doi: 10.1007/978-1-61779-092-8_10.

DOI:10.1007/978-1-61779-092-8_10

PMID:21461815

Abstract

Levels of telomerase activity can be an indicator of the proliferative potential of somatic cells and may serve as a diagnostic biomarker of malignancy. Telomeric repeat amplification protocol (TRAP) is a fast and sensitive PCR-based assay for detection and measurement of telomerase activity. Since its introduction, the TRAP assay has been widely used in cancer and aging studies. It provides a powerful alternative to other in vitro telomerase detection methods, and has been combined with other molecular techniques to screen telomerase inhibitors, and to study human malignancy and other diseases associated with telomere or telomerase dysfunction.

摘要

端粒酶活性水平可作为体细胞增殖潜力的指标，并可能用作恶性肿瘤的诊断生物标志物。端粒重复序列扩增法（TRAP）是一种基于聚合酶链反应（PCR）的快速且灵敏的检测方法，用于检测和测量端粒酶活性。自问世以来，TRAP检测法已广泛应用于癌症和衰老研究。它为其他体外端粒酶检测方法提供了有力的替代方案，并已与其他分子技术相结合，用于筛选端粒酶抑制剂，以及研究人类恶性肿瘤和其他与端粒或端粒酶功能障碍相关的疾病。