从毛丝鼠中分离和表征多态性微卫星位点。

Isolation and characterization of polymorphic microsatellite loci from Octodon degus.

机构信息

Department of Biology, University of Louisiana at Monroe, Monroe, LA 71209, USA Museum of Vertebrate Zoology, University of California, Berkeley, 3101 Valley Life Sciences Building, Berkeley, CA 94720, USA Facultad de Ciencias Biológicas and Center for Advanced Studies in Ecology and Biodiversity, Pontificia Universidad Católica de Chile, 6513677 Santiago, Chile.

出版信息

Mol Ecol Resour. 2009 May;9(3):999-1001. doi: 10.1111/j.1755-0998.2009.02536.x. Epub 2009 Feb 10.

DOI:10.1111/j.1755-0998.2009.02536.x

PMID:21564818

Abstract

Quantifying genetic kinship and parentage is critical to understanding the adaptive consequences of sociality. To measure fitness in a species with variable group structure, we isolated 14 microsatellite loci from Octodon degus, a semi-fossorial rodent endemic to Chile. The number of alleles per locus ranged from four to 14. Thirteen loci were in Hardy-Weinberg proportions, with values of observed heterozygosity ranging from 0.550 to 0.950. These markers provide the basis for future studies of the direct fitness consequences of sociality in O. degus.

摘要

量化遗传亲缘关系和亲子关系对于理解社会性的适应后果至关重要。为了衡量具有可变群体结构的物种的适应度，我们从智利特有的半穴居啮齿动物——毛丝鼠中分离出 14 个微卫星基因座。每个基因座的等位基因数从 4 到 14 不等。13 个基因座符合哈迪-温伯格平衡，观测杂合度值范围为 0.550 到 0.950。这些标记物为未来研究毛丝鼠的社会性对直接适应度的影响提供了基础。