人类细胞中 RNA 聚合酶 II 的快速转录速率。
Fast transcription rates of RNA polymerase II in human cells.
机构信息
Laboratory of Molecular Virology, International Centre for Genetic Engineering and Biotechnology (ICGEB), Padriciano 99, Trieste 34149, Italy.
出版信息
EMBO Rep. 2011 Dec 1;12(12):1280-5. doi: 10.1038/embor.2011.196.
Abstract
Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min(-1). In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min(-1) points towards a wide dynamic range of RNAPII velocities in living cells.
摘要
哺乳动物细胞中 RNA 聚合酶 II（RNAPII）延伸率的平均估计值在 1.3 到 4.3 kb/min 之间。在这项工作中，通过荧光 RNA 标记，可以在单细胞水平上检测到整合的人类免疫缺陷病毒 1 衍生载体的新生 RNA。在稳定状态下，测量到与聚合酶的最小密度相对应的恒定数量的 RNA，这些聚合酶的数量随时间的变化可以忽略不计。光漂白后荧光的恢复在几秒钟内完全完成，表明 RNA 生物发生的速度很高。计算得到的转录速率超过 50 kb/min，表明活细胞中 RNAPII 速度具有很宽的动态范围。
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