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大片段基因组序列的直接克隆。

Direct cloning of large genomic sequences.

出版信息

Nat Biotechnol. 2012 May 7;30(5):405-6. doi: 10.1038/nbt.2207.

Abstract

The discovery of an efficient mechanism of homologous recombination between two linear DNA substrates enables direct cloning of large genomic sequences.

摘要

该发现为两种线性 DNA 底物之间的同源重组提供了一种高效机制,从而实现了大片段基因组序列的直接克隆。

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本文引用的文献

1
Circular polymerase extension cloning of complex gene libraries and pathways.
PLoS One. 2009 Jul 30;4(7):e6441. doi: 10.1371/journal.pone.0006441.
2
Triggering cryptic natural product biosynthesis in microorganisms.
Org Biomol Chem. 2009 May 7;7(9):1753-60. doi: 10.1039/b821578b. Epub 2009 Mar 6.
3
Enzymatic assembly of DNA molecules up to several hundred kilobases.
Nat Methods. 2009 May;6(5):343-5. doi: 10.1038/nmeth.1318. Epub 2009 Apr 12.
4
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways.
Nucleic Acids Res. 2009 Feb;37(2):e16. doi: 10.1093/nar/gkn991. Epub 2008 Dec 12.
5
A one pot, one step, precision cloning method with high throughput capability.
PLoS One. 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008 Nov 5.
6
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.
Nat Methods. 2007 Mar;4(3):251-6. doi: 10.1038/nmeth1010. Epub 2007 Feb 11.
7
Big effects from small changes: possible ways to explore nature's chemical diversity.
Chembiochem. 2002 Jul 2;3(7):619-27. doi: 10.1002/1439-7633(20020703)3:7<619::AID-CBIC619>3.0.CO;2-9.

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