Nat Biotechnol. 2012 May 7;30(5):405-6. doi: 10.1038/nbt.2207.
The discovery of an efficient mechanism of homologous recombination between two linear DNA substrates enables direct cloning of large genomic sequences.
该发现为两种线性 DNA 底物之间的同源重组提供了一种高效机制,从而实现了大片段基因组序列的直接克隆。