比率型荧光肽传感器用于 Cd2+ 的高选择性检测。
Synthesis of a ratiometric fluorescent peptide sensor for the highly selective detection of Cd2+.
机构信息
Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology, School of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng 252059, PR China.
出版信息
Bioorg Med Chem Lett. 2012 Jun 15;22(12):4014-7. doi: 10.1016/j.bmcl.2012.04.088. Epub 2012 Apr 25.
A novel ratiometric fluorescent peptidyl chemosensor (Dansyl-Cys-Pro-Gly-Cys-Trp-NH(2), D-P5) for metal ions detection has been synthesized via Fmoc solid-phase peptide synthesis. The chemosensor exhibited a high selectivity for Cd(2+) over other metal ions including competitive transition and Group I and II metal ions in neutral pH. The fluorescence emission intensity of D-P5 was significantly enhanced in the presence of Cd(2+) by fluorescent resonance energy transfer (FRET) and chelation enhanced fluorescence (CHEF) effects. The binding stoichiometry, detection limit, binding affinity, reversibility and pH sensitivity of the sensor for Cd(2+) were investigated.
一种新型比率型荧光肽类化学传感器（Dansyl-Cys-Pro-Gly-Cys-Trp-NH(2)，D-P5）已经通过 Fmoc 固相肽合成法合成，用于金属离子检测。该化学传感器在中性 pH 条件下，对 Cd(2+)具有高度选择性，能够区分其他金属离子，包括竞争性过渡金属离子和Ⅰ族和Ⅱ族金属离子。D-P5 在存在 Cd(2+)的情况下，通过荧光共振能量转移（FRET）和螯合增强荧光（CHEF）效应，荧光发射强度显著增强。研究了该传感器对 Cd(2+)的结合比、检测限、结合亲和力、可逆性和 pH 敏感性。
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