[生物发光方法在医学中的应用前景]

[Perspectives of the use of bioluminescence methods in medicine].

作者信息

Gitel'zon I I, Sandalova T P

出版信息

Vestn Akad Med Nauk SSSR. 1990(9):31-5.

Abstract

Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.

摘要

本文讨论了生物发光分析在检测某些生物活性物质方面的开发与应用的重大进展。该方法的主要优点在于其高灵敏度、高特异性，以及操作简单、检测快速。现有方法可用于检测多种不同性质的物质：钙离子、三磷酸腺苷（ATP）、黄素单核苷酸（FMN）、烟酰胺腺嘌呤二核苷酸（NAD(P)）、长链醛、依赖ATP和NAD(P)的酶及其底物、许多外源性物质和抗生素以及诱变剂。生物发光方法可广泛应用于临床实验室诊断。