利用纳米孔单分子计数器检测 microRNA。
Detection of miRNAs with a nanopore single-molecule counter.
机构信息
Biological Engineering and Dalton Cardiovascular Research Center, University of Missouri, Columbia, MO 65211, USA.
出版信息
Expert Rev Mol Diagn. 2012 Jul;12(6):573-84. doi: 10.1586/erm.12.58.
Abstract
miRNAs are short noncoding RNA molecules that are important in regulating gene expression. Due to the correlation of their expression levels and various diseases, miRNAs are being investigated as potential biomarkers for molecular diagnostics. The fast-growing miRNA exploration demands rapid, accurate, low-cost miRNA detection technologies. This article will focus on two platforms of nanopore single-molecule approach that can quantitatively measure miRNA levels in samples from tissue and cancer patient plasma. Both nanopore methods are sensitive and specific, and do not need labeling, enzymatic reaction or amplification. In the next 5 years, the nanopore-based miRNA techniques will be improved and validated for noninvasive and early diagnosis of diseases.
摘要
miRNAs 是一类短的非编码 RNA 分子，在调节基因表达方面发挥着重要作用。由于它们的表达水平与各种疾病相关，miRNAs 被作为分子诊断的潜在生物标志物进行研究。miRNA 的快速发展需要快速、准确、低成本的 miRNA 检测技术。本文将重点介绍两种基于纳米孔单分子方法的平台，这些方法可以定量测量组织样本和癌症患者血浆中的 miRNA 水平。这两种纳米孔方法都具有高灵敏度和特异性，并且不需要标记、酶反应或扩增。在未来 5 年内，基于纳米孔的 miRNA 技术将得到改进和验证，用于疾病的非侵入性和早期诊断。
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