螺吡喃共轭的聚轮烷作为一种双重响应比色探测器。

Spiropyran-conjugated pluronic as a dual responsive colorimetric detector.

机构信息

Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea.

出版信息

Macromol Rapid Commun. 2012 Nov 23;33(22):1958-63. doi: 10.1002/marc.201200416. Epub 2012 Aug 21.

DOI:10.1002/marc.201200416

PMID:22907706

Abstract

Novel spiropyran-conjugated Pluronic [polyethylene oxide (PEO)-b-polypropylene oxide (PPO)-b-polyethylene oxide (PEO)] micelles are developed as a new colorimetric detector showing photo- or thermo-switchable behavior. Facile conjugation of spiropyran to Pluronic was confirmed by (1)H NMR, UV-Vis, and Fluorescence spectroscopy. A switchable photoluminescence is found depending on the irradiation with either UV or visible light, and temperature resulting from structural isomerization of spiropyran between spiropyran (SP) and merocyanine (MC) form. Cytotoxicity of the spiropyran-conjugated Pluronic (SP-PL) was evaluated following an MTT assay, whereas photo responsiveness of spiropyran within the micelles was determined by confocal laser scanning microscopy.

摘要

新型螺吡喃偶联型 Pluronic [聚环氧乙烷（PEO）-b-聚丙二醇（PPO）-b-聚环氧乙烷（PEO）] 胶束被开发为一种新型比色探测器，具有光或热开关行为。通过 1H NMR、UV-Vis 和荧光光谱证实了螺吡喃与 Pluronic 的简便偶联。发现螺吡喃与开链形式（MC）之间的结构互变异构导致光致发光和温度的可切换，这取决于用紫外光或可见光的辐照。通过 MTT 测定法评估了螺吡喃偶联 Pluronic（SP-PL）的细胞毒性，而通过共聚焦激光扫描显微镜确定了胶束中螺吡喃的光响应性。