Trmt61B 是一种甲基转移酶，负责人类线粒体 tRNA 第 58 位的 1-甲基腺苷。
Trmt61B is a methyltransferase responsible for 1-methyladenosine at position 58 of human mitochondrial tRNAs.
机构信息
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
出版信息
RNA. 2012 Dec;18(12):2269-76. doi: 10.1261/rna.035600.112. Epub 2012 Oct 24.
In human mitochondria, 1-methyladenosine (m¹A) occurs at position 58 of tRNA(Leu(UUR)). In addition, partial m¹A58 modifications have been found in human mitochondrial tRNA(Lys) and tRNA(Ser(UCN)). We identified human Trmt61B, which encodes a mitochondria-specific tRNA methyltransferase responsible for m¹A58 in these three tRNAs. Trmt61B is dominantly localized to the mitochondria. m¹A58 formation in human mitochondrial tRNA(Leu(UUR)) could be reconstituted in vitro using recombinant Trmt61B in the presence of Ado-Met as a methyl donor. Unlike the cytoplasmic tRNA m¹A58 methyltransferase that consists of an α2β2 heterotetramer formed by Trmt61A and Trmt6, Trmt61B formed a homo-oligomer (presumably a homotetramer) that resembled the bacterial homotetrameric m¹A58 methyltransferase. The bacterial origin of Trmt61B is supported by the results of the phylogenetic analysis.
在人类线粒体中，1-甲基腺嘌呤（m¹A）位于 tRNA（Leu（UUR））的第 58 位。此外，在人类线粒体 tRNA（Lys）和 tRNA（Ser（UCN））中发现了部分 m¹A58 修饰。我们鉴定了人类 Trmt61B，它编码一种负责这三种 tRNA 中 m¹A58 的线粒体特异性 tRNA 甲基转移酶。Trmt61B 主要定位于线粒体。使用重组 Trmt61B 在 Ado-Met 作为甲基供体的存在下，可在体外重新构建人线粒体 tRNA（Leu（UUR））中的 m¹A58 形成。与由 Trmt61A 和 Trmt6 形成的α2β2 异四聚体组成的细胞质 tRNA m¹A58 甲基转移酶不同，Trmt61B 形成了同源寡聚体（推测为四聚体），类似于细菌同源四聚体 m¹A58 甲基转移酶。系统发育分析的结果支持 Trmt61B 的细菌起源。
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