评价 brilliance MRSA 2 琼脂在临床样本中检测耐甲氧西林金黄色葡萄球菌的性能。
Evaluation of brilliance MRSA 2 agar for detection of methicillin-resistant Staphylococcus aureus in clinical samples.
机构信息
Laboratory for Microbiology and Infection Control, Amphia Hospital, Breda, The Netherlands.
出版信息
J Clin Microbiol. 2013 Mar;51(3):1026-7. doi: 10.1128/JCM.02995-12. Epub 2013 Jan 2.
Abstract
We compared 2 chromogenic media (Oxoid Brilliance MRSA 2 agar [Thermo Fisher Scientific] and MRSA-ID [bioMérieux]) for the detection of methicillin-resistant Staphylococcus aureus (MRSA) in 1,368 hospital samples. For both media, broth enrichment was essential to obtain satisfactory diagnostic performance. Although with direct cultures only, the diagnostic performance (particularly sensitivity) of Brilliance MRSA 2 agar appears better than that of MRSA-ID, no difference in sensitivity or specificity between the media was detected after inclusion of an enrichment step.
摘要
我们比较了两种显色培养基（Oxoid Brilliance MRSA 2 琼脂[赛默飞世尔科技]和 MRSA-ID[生物梅里埃]）用于检测 1368 份医院样本中的耐甲氧西林金黄色葡萄球菌（MRSA）。对于这两种培养基，肉汤增菌对于获得满意的诊断性能都是必不可少的。虽然仅使用直接培养，但是 Brilliance MRSA 2 琼脂的诊断性能（特别是敏感性）似乎优于 MRSA-ID，但是在加入增菌步骤后，两种培养基之间的敏感性或特异性没有差异。
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本文引用的文献
1
Evaluation of the impact of direct plating, broth enrichment, and specimen source on recovery and diversity of methicillin-resistant Staphylococcus aureus isolates among HIV-infected outpatients.
J Clin Microbiol. 2011 Dec;49(12):4126-30. doi: 10.1128/JCM.05323-11. Epub 2011 Oct 12.
2
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3
A blinded comparison of three laboratory protocols for the identification of patients colonized with methicillin-resistant Staphylococcus aureus.
Infect Control Hosp Epidemiol. 2001 Mar;22(3):152-6. doi: 10.1086/501882.
4
Effect of broth enrichment cultures on ability to detect carriage of Staphylococcus aureus.
Antimicrob Agents Chemother. 1995 Sep;39(9):2169. doi: 10.1128/AAC.39.9.2169.
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