与麻风病患者有家庭接触者的抗 PGL1 唾液 IgA/IgM、血清 IgG/IgM 和鼻分枝杆菌 DNA。

Anti-PGL1 salivary IgA/IgM, serum IgG/IgM, and nasal Mycobacterium leprae DNA in individuals with household contact with leprosy.

机构信息

Department of Clinical Analysis and Toxicology, Faculty of Pharmacy, Universidade Federal do Ceará, Rua Capitão Francisco Pedro, 1210, CEP 60430-370 Fortaleza, Ceará, Brazil.

出版信息

Int J Infect Dis. 2013 Nov;17(11):e1005-10. doi: 10.1016/j.ijid.2013.05.011. Epub 2013 Jul 16.

DOI:10.1016/j.ijid.2013.05.011

PMID:23871279

Abstract

OBJECTIVES

Leprosy household contacts represent a group at high risk of developing the disease. The aim of this study was to detect Mycobacterium leprae subclinical infection in this group through serological and molecular parameters.

METHODS

Serum anti-PGL1 IgG/IgM and salivary anti-PGL1 IgA/IgM was investigated using an ELISA, and nasal carriage of M. leprae DNA was detected by PCR, in leprosy household contacts of paucibacillary (PB) and multibacillary (MB) household leprosy patients (n=135), their index cases (n=30), and in persons living in a low endemic city (n=17).

RESULTS

Salivary anti-PGL1 IgA and IgM and serum anti-PGL1 IgG showed good correlation comparing contacts and index cases (p<0.01, p<0.005, and p<0.0001, respectively). This was not observed for serum anti-PGL1 IgM (p>0.05). A high frequency of anti-PGL1 IgM positivity was found in IgG-negative samples (p<0.0001). For IgG-positive samples, IgM antibodies were also positive in most of the samples. None of the 17 volunteers living in a low endemic city presented seropositivity for IgG; however, two of them showed positivity for anti-PGL1 IgM. M. leprae DNA was found in the nasal swabs of nine out of the 85 MB household leprosy contacts (10.6%) and in three out of the 50 PB household leprosy contacts (6.0%).

CONCLUSION

We strongly suggest that serum IgG/IgM and salivary anti-PGL1 IgA/IgM measurements are used to follow leprosy household contacts.

摘要

目的

麻风病家庭接触者代表了一组罹患该病的高风险人群。本研究旨在通过血清学和分子参数检测该人群中麻风分枝杆菌亚临床感染。

方法

采用 ELISA 法检测血清抗 PGL1 IgG/IgM 和唾液抗 PGL1 IgA/IgM，采用 PCR 法检测鼻拭子中麻风分枝杆菌 DNA，检测麻风病家庭接触者（包括少菌型和多菌型家庭麻风病患者的家庭接触者，n=135）、其索引病例（n=30）和生活在低流行城市的人员（n=17）。

结果

唾液抗 PGL1 IgA 和 IgM 以及血清抗 PGL1 IgG 显示接触者和索引病例之间具有良好的相关性（p<0.01、p<0.005 和 p<0.0001）。但血清抗 PGL1 IgM 未显示出相关性（p>0.05）。在 IgG 阴性样本中发现抗 PGL1 IgM 阳性率较高（p<0.0001）。对于 IgG 阳性样本，IgM 抗体也在大多数样本中呈阳性。生活在低流行城市的 17 名志愿者中均未出现 IgG 血清阳性；然而，其中 2 人抗 PGL1 IgM 呈阳性。在 85 例多菌型家庭麻风病接触者的鼻拭子中发现了 9 例（10.6%）和 50 例少菌型家庭麻风病接触者中的 3 例（6.0%）有麻风分枝杆菌 DNA。