孕激素受体基因多态性与男性不育及 ICSI 临床结局的关系。
Association of progesterone receptor gene polymorphism with male infertility and clinical outcome of ICSI.
机构信息
National Institute for Research in Reproductive Health (ICMR), J. M. Street, Parel, Mumbai, 400012, Maharashtra, India.
出版信息
J Assist Reprod Genet. 2013 Sep;30(9):1133-9. doi: 10.1007/s10815-013-0074-2. Epub 2013 Aug 11.
PURPOSE
To investigate the association of Progesterone Receptor (PR) gene variations and male infertility
METHODS
DNA extraction, PCR and sequencing of PR gene, PROGINS insertion by PCR. Association of the variations with seminal parameters and outcomes of ICSI.
RESULTS
Four known SNPs in the PR gene were identified in the study of which three (rs3740753, rs1042838, rs104283) were co-inherited and in complete linkage disequilibrium with the PROGINS Alu insertion. There were no differences in their frequencies between fertile and infertile males. The rs2020880 was found at a very low frequency only in the controls but not in the infertile subjects. The sperm counts, fertilization rate, embryo quality or pregnancy rates were not different in individuals with or without PROGINS allele.
CONCLUSION
PR gene alterations are not associated with male infertility or ICSI outcome.
目的
研究孕激素受体（PR）基因变异与男性不育的关系。
方法
提取 DNA，对 PR 基因进行 PCR 和测序，采用 PCR 检测 PROGINS 插入。分析变异与精液参数及 ICSI 结局的关系。
结果
在 PR 基因的研究中发现了 4 个已知的 SNP，其中 3 个（rs3740753、rs1042838、rs104283）是共遗传的，与 PROGINS Alu 插入完全连锁不平衡。在不育和正常男性中，它们的频率没有差异。rs2020880 仅在对照组中以非常低的频率存在，而在不育组中不存在。具有或不具有 PROGINS 等位基因的个体的精子计数、受精率、胚胎质量或妊娠率没有差异。
结论
PR 基因改变与男性不育或 ICSI 结局无关。
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