机械压迫力抑制脂肪干细胞的脂肪生成。
Mechanical compressive force inhibits adipogenesis of adipose stem cells.
机构信息
State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, China.
出版信息
Cell Prolif. 2013 Oct;46(5):586-94. doi: 10.1111/cpr.12053. Epub 2013 Aug 29.
Abstract
OBJECTIVE
The aim of this study was to investigate effects of mechanical compressive force on differentiation of adipose-derived stem cells (ASCs) in vitro.
MATERIALS AND METHODS
Mice ASCs were treated with compressive force (2000 με, 1 Hz) for 2 or 6 h after adipogenic induction for 3 days, then oil red O staining was used to examine oil droplet-filled cells. Adipogenic genes, PPAR-γ1 and APN, were examined by real-time PCR and immunofluorescence (IF) staining was performed to test expression of de-PPAR-γ and ph-PPAR-γ at the protein level.
RESULTS
Our data showed that mechanical compressive force reduced numbers of oil droplet-filled cells, and down-regulated mRNA levels of both PPAR-γ1 and APN and protein level of PPAR-γ, in ASCs.
CONCLUSIONS
In culture medium containing adipogenic stimuli, mechanical compressive force inhibited adipogenesis of ASCs.
摘要
目的
本研究旨在探讨体外机械压缩力对脂肪干细胞（ASCs）分化的影响。
材料与方法
在诱导脂肪生成 3 天后，用 2000 με、1 Hz 的压缩力处理小鼠 ASCs 2 或 6 h，然后用油红 O 染色检测充满油滴的细胞。通过实时 PCR 检测脂肪生成基因 PPAR-γ1 和 APN，通过免疫荧光（IF）染色检测去 PPAR-γ 和 ph-PPAR-γ 在蛋白质水平的表达。
结果
我们的数据表明，机械压缩力减少了充满油滴的细胞数量，并下调了 ASCs 中 PPAR-γ1 和 APN 的 mRNA 水平以及 PPAR-γ 的蛋白水平。
结论
在含有脂肪生成刺激物的培养基中，机械压缩力抑制了 ASCs 的脂肪生成。
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