分化的 Troy+主细胞作为储备干细胞，可产生胃上皮的所有谱系。
Differentiated Troy+ chief cells act as reserve stem cells to generate all lineages of the stomach epithelium.
机构信息
Hubrecht Institute for Developmental Biology and Stem Cell Research & University Medical Centre Utrecht, 3584 CT Utrecht, the Netherlands; Department of General, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, University of Dresden, 01307 Dresden, Germany.
出版信息
Cell. 2013 Oct 10;155(2):357-68. doi: 10.1016/j.cell.2013.09.008.
Proliferation of the self-renewing epithelium of the gastric corpus occurs almost exclusively in the isthmus of the glands, from where cells migrate bidirectionally toward pit and base. The isthmus is therefore generally viewed as the stem cell zone. We find that the stem cell marker Troy is expressed at the gland base by a small subpopulation of fully differentiated chief cells. By lineage tracing with a Troy-eGFP-ires-CreERT2 allele, single marked chief cells are shown to generate entirely labeled gastric units over periods of months. This phenomenon accelerates upon tissue damage. Troy(+) chief cells can be cultured to generate long-lived gastric organoids. Troy marks a specific subset of chief cells that display plasticity in that they are capable of replenishing entire gastric units, essentially serving as quiescent "reserve" stem cells. These observations challenge the notion that stem cell hierarchies represent a "one-way street."
胃体的自我更新上皮细胞的增殖几乎只发生在腺体的峡部，细胞从那里向胃小凹和胃底双向迁移。因此，峡部通常被认为是干细胞区。我们发现，干细胞标记物 Troy 由一小部分完全分化的主细胞在腺体底部表达。通过 Troy-eGFP-ires-CreERT2 等位基因的谱系追踪，单个标记的主细胞被证明可以在数月的时间内产生完全标记的胃单位。这种现象在组织损伤时会加速。Troy(+)主细胞可被培养以产生长期存在的胃类器官。Troy 标记了主细胞的一个特定亚群，它们具有可塑性，因为它们能够补充整个胃单位，实质上充当静止的“储备”干细胞。这些观察结果挑战了干细胞层次结构代表“单行道”的观点。
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