基于 CdSe 和 DNA 在 TiO2 薄膜电极上组装的对氨基苯酚光电化学生物传感器。
A photoelectrochemical biosensor for o-aminophenol based on assembling of CdSe and DNA on TiO2 film electrode.
机构信息
School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Luoyu Road 1037, Wuhan 430074, PR China.
出版信息
Biosens Bioelectron. 2014 Mar 15;53:301-4. doi: 10.1016/j.bios.2013.09.073. Epub 2013 Oct 9.
A novel photoelectrochemical (PEC) biosensing platform was constructed by assembling CdSe quantum dots (QDs) and DNA on liquid phase deposited TiO2 (DNA-CdSe/TiO2) film electrode. The transmission electron microscopy (TEM), scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis indicated that CdSe QDs were homogeneously assembled on TiO2 film. The UV-visible diffuse reflectance spectra (DRS) showed that CdSe and DNA could effectively enhance the absorption of TiO2 film to visible light. The obtained electrode showed a sensitive PEC response to o-aminophenol (OAP) under visible light irradiation. Due to the interaction between DNA and OAP, the response of OAP was improved by DNA immobilized on the sensing film. Under optimized conditions, the photocurrent was linearly proportional to OAP in the concentration range from 4.0 × 10(-7) to 2.7 × 10(-5) mol L(-1), with a detection limit (3S/N) of 8.0 × 10(-8) mol L(-1). The novel strategy could provide a fast and sensitive method for OAP determination.
构建了一种新型的光电化学（PEC）生物传感平台，通过将CdSe 量子点（QDs）和 DNA 组装在液相沉积的 TiO2（DNA-CdSe/TiO2）薄膜电极上。透射电子显微镜（TEM）、扫描电子显微镜（SEM）和 X 射线衍射（XRD）分析表明，CdSe QDs 均匀地组装在 TiO2 薄膜上。紫外-可见漫反射光谱（DRS）表明，CdSe 和 DNA 可以有效地增强 TiO2 薄膜对可见光的吸收。所得电极在可见光照射下对邻氨基酚（OAP）表现出灵敏的 PEC 响应。由于 DNA 与 OAP 之间的相互作用，固定在传感膜上的 DNA 提高了 OAP 的响应。在优化条件下，光电流在 4.0×10（-7）至 2.7×10（-5）mol L（-1）的浓度范围内与 OAP 呈线性关系，检测限（3S/N）为 8.0×10（-8）mol L（-1）。该新策略可为 OAP 的测定提供快速灵敏的方法。
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