抗菌肽 anoplin 的新特性。
Novel properties of antimicrobial peptide anoplin.
机构信息
Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, 165 02 Prague 6, Czech Republic; Department of Biochemistry and Microbiology, Institute of Chemical Technology Prague, Technická 5, 165 21 Prague 6, Czech Republic.
Institute of Experimental Botany, Czech Academy of Science, Rozvojová 313, 165 02 Prague 6, Czech Republic.
出版信息
Biochem Biophys Res Commun. 2014 Feb 21;444(4):520-4. doi: 10.1016/j.bbrc.2014.01.097. Epub 2014 Jan 25.
Antimicrobial decapeptide anoplin was tested for its antifungal activity against plant pathogen Leptosphaeria maculans and protection of Brassica napus plants from disease. To reveal the mode of action of the peptide, a natural form of anoplin amidated on C-terminus (ANP-NH2), and its carboxylated analog (ANP-OH) were used in the study. We demonstrated strong antifungal activity of anoplin in vitro regardless C-terminus modification. In addition we show that both ANP-NH2 and ANP-OH induce expression of defence genes in B. napus and protects plants from L. maculans infection. The results indicate that the amidation of anoplin is not essential for its antifungal and plant defence stimulating activities.
抗菌十肽 anoplin 已被测试其抗真菌活性，针对植物病原菌 Leptosphaeria maculans 并保护油菜植物免受疾病侵害。为了揭示该肽的作用模式，使用了 C 末端酰胺化的天然形式的 anoplin（ANP-NH2）及其羧基化类似物（ANP-OH）进行了研究。我们证明了 anoplin 在体外具有很强的抗真菌活性，而与 C 末端修饰无关。此外，我们还表明，ANP-NH2 和 ANP-OH 均可诱导油菜中的防御基因表达，并保护植物免受 L. maculans 的感染。结果表明，anoplin 的酰胺化对于其抗真菌和植物防御刺激活性不是必需的。
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