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二硫苏糖醇:一种可抑制叶片提取物和分离的叶绿体中葡萄糖-6-磷酸脱氢酶活性的物质。

Dithiothreitol: An inhibitor of glucose-6-phosphate-dehydrogenase activity in leaf extracts and isolated chloroplasts.

机构信息

Department of Botany, University of Queensland, St. Lucia, Queensland, Australia.

出版信息

Planta. 1972 Sep;106(3):273-7. doi: 10.1007/BF00388105.

Abstract

The activity of glucose-6-phosphate dehydrogenase (G-6-P-DH; D-glucose 6-phosphate: NADP oxidoreductase, EC 1.1.1.49) in leaf extracts of barley and spinach can be decreased 20-35% by incubation of the leaf extracts with dithiothreitol (DTT). This inhibition is complete within 2 min at 0°C and is reversible. The DTT-inhibited portion of G-6-P-DH activity in leaf extracts is probably that portion of leaf enzyme inhibited during illumination, and evidence has been obtained that this activity is located in the chloroplasts.

摘要

葡萄糖-6-磷酸脱氢酶(G-6-P-DH;D-葡萄糖 6-磷酸:NADP 氧化还原酶,EC 1.1.1.49)在大麦和菠菜叶提取物中的活性可被二硫苏糖醇(DTT)降低 20-35%。这种抑制在 0°C 下 2 分钟内完全,并且是可逆的。叶提取物中 G-6-P-DH 活性的 DTT 抑制部分可能是在光照期间被抑制的叶酶部分,并且已经获得了该活性位于叶绿体中的证据。

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