一种用于选择性灵敏检测硫酚的香豆素基荧光探针及其应用。
A coumarin-based fluorescent probe for selective and sensitive detection of thiophenols and its application.
作者信息
Li Jun, Zhang Chun-Fang, Yang Shu-Hou, Yang Wen-Chao, Yang Guang-Fu
机构信息
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , Wuhan 430079, P.R. China.
出版信息
Anal Chem. 2014 Mar 18;86(6):3037-42. doi: 10.1021/ac403885n. Epub 2014 Mar 3.
The development of probes for specific thiophenol detection is of great importance, due to the toxicity of thiophenols and their derivatives in the environment. In the present study, a novel fluorescent probe was rationally designed for detecting thiophenols via an intramolecular charge transfer mechanism. The developed probe selectively and sensitively distinguished thiophenols from aliphatic thiols. It displayed a large Stokes shift (145 nm) and >280-fold fluorescence enhancement. Moreover, the new probe not only displayed excellent cell permeability for the successful detection of thiophenol in HEK293 cells but also quantitatively measured thiophenols in water samples with good recovery (more than 90%), indicating that it has promising prospects for application for thiophenol sensing in environmental and biological sciences.
由于苯硫酚及其衍生物在环境中的毒性，开发用于特异性检测苯硫酚的探针具有重要意义。在本研究中，通过分子内电荷转移机制合理设计了一种新型荧光探针用于检测苯硫酚。所开发的探针能够选择性且灵敏地将苯硫酚与脂肪族硫醇区分开来。它表现出较大的斯托克斯位移（145 nm）和>280倍的荧光增强。此外，这种新型探针不仅对在HEK293细胞中成功检测苯硫酚表现出优异的细胞渗透性，还能对水样中的苯硫酚进行定量测定，回收率良好（超过90%），表明其在环境和生物科学中用于苯硫酚传感具有广阔的应用前景。
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