辣根过氧化物酶模拟 DNA zyme 增强 CdTe 量子点-H₂O₂的化学发光。
Enhanced chemiluminescence of CdTe quantum dots-H₂O₂ by horseradish peroxidase-mimicking DNAzyme.
机构信息
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2014 May 5;125:228-33. doi: 10.1016/j.saa.2014.01.111. Epub 2014 Feb 5.
In this study, it was found that horseradish peroxidase (HRP)-mimicking DNAzyme could effectively enhance the CL emission of CdTe quantum dots (QDs)-H2O2 system, whereas HRP could not enhance the CL intensity. The CL enhancement mechanism was investigated, and the CL enhancement was supposed to originate from the catalysis of HRP-mimicking DNAzyme on the CL reaction between CdTe QDs and H2O2. Meantime, compared with CdTe QDs-H2O2 CL system, H2O2 concentration was markedly decreased in QDs-H2O2-HRP-mimicking DNAzyme CL system, improving the stability of QDs-H2O2 CL system. The QDs-based CL system was used to detect sensitively CdTe QDs and HRP-mimicking DNAzyme (as biologic labels). This work gives a path for enhancing CL efficiency of QDs system, and will be helpful to promote the step of QDs application in various fields such as bioassay and trace detection of analyte.
在这项研究中，发现辣根过氧化物酶（HRP）模拟 DNA 酶可以有效地增强 CdTe 量子点（QDs）-H2O2 体系的 CL 发射，而 HRP 不能增强 CL 强度。研究了 CL 增强机制，CL 增强被认为源于 HRP 模拟 DNA 酶对 CdTe QDs 和 H2O2 之间 CL 反应的催化。同时，与 CdTe QDs-H2O2 CL 体系相比，在 QDs-H2O2-HRP 模拟 DNA 酶 CL 体系中 H2O2 浓度明显降低，提高了 QDs-H2O2 CL 体系的稳定性。基于 QDs 的 CL 体系被用于灵敏地检测 CdTe QDs 和 HRP 模拟 DNA 酶（作为生物标记物）。这项工作为增强 QDs 体系的 CL 效率提供了一条途径，将有助于推动 QDs 在生物测定和分析物痕量检测等各个领域的应用。
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