赤道几内亚HIV血清阳性患者中隐孢子虫亚型分离株的鉴定
Identification of Cryptosporidium subtype isolates from HIV-seropositive patients in Equatorial Guinea.
作者信息
Blanco María A, Montoya Ana, Iborra Asunción, Fuentes Isabel
机构信息
Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
Centro de Referencia para el Control de Endemias (Guinea Ecuatorial) - Centro Nacional de Medicina Tropical, ISCIII, Madrid, Spain.
出版信息
Trans R Soc Trop Med Hyg. 2014 Sep;108(9):594-6. doi: 10.1093/trstmh/tru108. Epub 2014 Jul 4.
BACKGROUND
Cryptosporidium spp. are enteric parasites that infect humans and animals. In immunocompromised patients infection can be fatal. This study was conducted to identify sub-populations of Cryptosporidium hominis and C. parvum isolates from HIV-seropositive patients in Equatorial Guinea.
METHODS
In a previous study conducted in Equatorial Guinea, faecal samples from 171 HIV patients with gastrointestinal symptoms were analyzed. Of these, 13 and 17 were positive for C. hominis and C. parvum, respectively. The isolates were characterized using gp60 gene analysis.
RESULTS
The gp60 gene could only be detected in 57% (17/30) of cases (10 C. parvum and 7 C. hominis). Three C. hominis (Ia, Ib and Id) and two C. parvum (IIc and IIe) subtype families were detected, including several subtypes.
CONCLUSIONS
The study identified a high diversity of Cryptosporidium subtypes, suggesting that anthroponotic transmission plays an important role in the epidemiology of Cryptosporidium spp. in HIV-seropositive patients in Equatorial Guinea.
背景
隐孢子虫属是感染人类和动物的肠道寄生虫。在免疫功能低下的患者中，感染可能是致命的。本研究旨在鉴定赤道几内亚HIV血清阳性患者中微小隐孢子虫和 parvum 隐孢子虫分离株的亚群。
方法
在赤道几内亚进行的一项先前研究中，分析了171名有胃肠道症状的HIV患者的粪便样本。其中，微小隐孢子虫和 parvum 隐孢子虫分别有13例和17例呈阳性。使用gp60基因分析对分离株进行特征鉴定。
结果
仅在57%（17/30）的病例中检测到gp60基因（10例 parvum 隐孢子虫和7例微小隐孢子虫）。检测到3个微小隐孢子虫（Ia、Ib和Id）和2个 parvum 隐孢子虫（IIc和IIe）亚型家族，包括几种亚型。
结论
该研究发现隐孢子虫亚型具有高度多样性，表明人传人在赤道几内亚HIV血清阳性患者隐孢子虫属的流行病学中起重要作用。
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