八种猴出血热病毒的每种次要结构蛋白在功能上都很重要。
Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important.
作者信息
Vatter Heather A, Di Han, Donaldson Eric F, Baric Ralph S, Brinton Margo A
机构信息
Department of Biology, Georgia State University, Atlanta, GA 30303, United States.
Department of Epidemiology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States.
出版信息
Virology. 2014 Aug;462-463:351-62. doi: 10.1016/j.virol.2014.06.001. Epub 2014 Jul 16.
The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication characteristics similar to those of the parental virus. A subgenomic mRNA was identified for the SHFV ORF previously identified as 2b. As an initial means of analyzing the functional relevance of each of the SHFV minor structural proteins, a set of mutant infectious clones was generated, each with the start codon of one minor structural protein ORF mutated. Different phenotypes were observed for each ortholog of the pairs of minor glycoproteins and all of the eight minor structural proteins were required for the production of infectious extracellular virus indicating that the duplicated sets of SHFV minor structural proteins are not functionally redundant.
猴出血热病毒（SHFV）基因组与动脉炎病毒科其他成员的基因组不同，它编码两组相邻的四个次要结构蛋白开放阅读框（ORF）。构建了一个稳定的、全长的、有感染性的SHFV-LVR cDNA克隆。从该克隆产生的病毒具有与亲本病毒相似的复制特性。为先前鉴定为2b的SHFV ORF鉴定出一个亚基因组mRNA。作为分析每个SHFV次要结构蛋白功能相关性的初步手段，生成了一组突变感染性克隆，每个克隆的一个次要结构蛋白ORF的起始密码子发生突变。对于次要糖蛋白对的每个直系同源物观察到不同的表型，并且产生有感染性的细胞外病毒需要所有八个次要结构蛋白，这表明SHFV次要结构蛋白的重复组在功能上并非冗余。
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