普洛沃克斯主动瓣膜上生物膜的形成：通过Illumina双端RNA测序、荧光原位杂交和共聚焦激光扫描显微镜分析其组成和向内生长情况。
Biofilm formation on the Provox ActiValve: Composition and ingrowth analyzed by Illumina paired-end RNA sequencing, fluorescence in situ hybridization, and confocal laser scanning microscopy.
作者信息
Timmermans Adriana J, Harmsen Hermie J M, Bus-Spoor Carien, Buijssen Kevin J D A, van As-Brooks Corina, de Goffau Marcus C, Tonk Rudi H, van den Brekel Michiel W M, Hilgers Frans J M, van der Laan Bernard F A M
机构信息
Department of Head and Neck Oncology and Surgery, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
Department of Medical Microbiology, University of Groningen, University Medical Center, Groningen, Groningen, The Netherlands.
出版信息
Head Neck. 2016 Apr;38 Suppl 1:E432-40. doi: 10.1002/hed.24014. Epub 2015 Jul 14.
BACKGROUND
The most frequent cause of voice prosthesis failure is microbial biofilm formation on the silicone valve, leading to destruction of the material and transprosthetic leakage. The Provox ActiValve valve is made of fluoroplastic, which should be insusceptible to destruction. The purpose of this study was to determine if fluoroplastic is insusceptible to destruction by Candida species.
METHODS
Thirty-three dysfunctional Provox ActiValves (collected 2011-2013). Biofilm analysis was performed with Illumina paired-end sequencing (IPES), assessment of biofilm-material interaction with fluorescence in situ hybridization (FISH), and confocal laser scanning microscopy (CLSM).
RESULTS
IPES (n = 10) showed that Candida albicans and Candida tropicalis are dominant populations on fluoroplastic and silicone. Microbial diversity is significantly lower on fluoroplastic. Lactobacillus gasseri is the prevalent bacterial strain on most voice prostheses. FISH and CLSM (n = 23): in none of the cases was ingrowth of Candida species present in the fluoroplastic.
CONCLUSION
Fluoroplastic material of Provox ActiValve seems insusceptible to destruction by Candida species, which could help improve durability of voice prostheses. © 2015 Wiley Periodicals, Inc. Head Neck 38: E432-E440, 2016.
背景
语音假体失效最常见的原因是硅胶瓣膜上形成微生物生物膜，导致材料破坏和假体渗漏。Provox ActiValve瓣膜由氟塑料制成，应不易被破坏。本研究的目的是确定氟塑料是否不易被念珠菌属破坏。
方法
33个功能失调的Provox ActiValve瓣膜（于2011年至2013年收集）。采用Illumina双端测序（IPES）进行生物膜分析，通过荧光原位杂交（FISH）和共聚焦激光扫描显微镜（CLSM）评估生物膜与材料的相互作用。
结果
IPES（n = 10）显示白色念珠菌和热带念珠菌是氟塑料和硅胶上的优势菌群。氟塑料上的微生物多样性显著较低。加氏乳杆菌是大多数语音假体上的优势菌株。FISH和CLSM（n = 23）：在所有病例中，氟塑料均未出现念珠菌属的向内生长。
结论
Provox ActiValve瓣膜的氟塑料材料似乎不易被念珠菌属破坏，这可能有助于提高语音假体的耐用性。© 2015威利期刊公司。头颈外科38：E432 - E440，2016年。
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