通过基质辅助激光解吸电离飞行时间质谱法鉴定具核梭杆菌亚种
Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
作者信息
Nie Shuping, Tian Baoyu, Wang Xiaowei, Pincus David H, Welker Martin, Gilhuley Kathleen, Lu Xuedong, Han Yiping W, Tang Yi-Wei
机构信息
Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA.
Department of Periodontics, Case Western Reserve University, Cleveland, Ohio, USA.
出版信息
J Clin Microbiol. 2015 Apr;53(4):1399-402. doi: 10.1128/JCM.00239-15. Epub 2015 Feb 4.
Abstract
We explored the use of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification of Fusobacterium nucleatum subspecies. MALDI-TOF MS spectra of five F. nucleatum subspecies (animalis, fusiforme, nucleatum, polymorphum, and vincentii) were analyzed and divided into four distinct clusters, including subsp. animalis, nucleatum, polymorphum, and fusiforme/vincentii. MALDI-TOF MS with the modified SARAMIS database further correctly identified 28 of 34 F. nucleatum clinical isolates to the subspecies level.
摘要
我们探索了使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）来鉴定具核梭杆菌亚种。分析了五个具核梭杆菌亚种（动物亚种、梭形亚种、具核亚种、多形亚种和文森亚种）的MALDI-TOF MS谱图，并将其分为四个不同的簇，包括动物亚种、具核亚种、多形亚种以及梭形/文森亚种。使用经过修改的SARAMIS数据库的MALDI-TOF MS进一步将34株具核梭杆菌临床分离株中的28株准确鉴定到亚种水平。
相似文献
1
Fusobacterium nucleatum subspecies identification by matrix-assisted laser desorption ionization-time of flight mass spectrometry.
J Clin Microbiol. 2015 Apr;53(4):1399-402. doi: 10.1128/JCM.00239-15. Epub 2015 Feb 4.
2
Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies.
J Clin Microbiol. 2010 Feb;48(2):545-53. doi: 10.1128/JCM.01631-09. Epub 2009 Dec 2.
3
Fusobacterium nucleatum subsp. fusiforme subsp. nov. and Fusobacterium nucleatum subsp. animalis subsp. nov. as additional subspecies within Fusobacterium nucleatum.
Int J Syst Bacteriol. 1992 Apr;42(2):296-8. doi: 10.1099/00207713-42-2-296.
4
Genome-Based Reclassification of Fusobacterium nucleatum Subspecies at the Species Level.
Curr Microbiol. 2017 Oct;74(10):1137-1147. doi: 10.1007/s00284-017-1296-9. Epub 2017 Jul 7.
5
Detection of Fusobacterium nucleatum subspecies in the saliva of pre-colorectal cancer patients, using tandem mass spectrometry.
Arch Oral Biol. 2022 Feb;134:105337. doi: 10.1016/j.archoralbio.2021.105337. Epub 2021 Dec 14.
6
Genome sequence of Fusobacterium nucleatum subspecies polymorphum - a genetically tractable fusobacterium.
PLoS One. 2007 Aug 1;2(7):e659. doi: 10.1371/journal.pone.0000659.
7
Draft genome sequence of Fusobacterium nucleatum subsp. fusiforme ATCC 51190T.
J Bacteriol. 2012 Oct;194(19):5445-6. doi: 10.1128/JB.01138-12.
8
Fusobacterium nucleatum subsp. fusiforme Gharbia and Shah 1992 is a later synonym of Fusobacterium nucleatum subsp. vincentii Dzink et al. 1990.
Curr Microbiol. 2013 Apr;66(4):414-7. doi: 10.1007/s00284-012-0289-y. Epub 2012 Dec 24.
9
Genotypic and phenotypic characterization of fusobacteria from Chinese and European patients with inflammatory periodontal diseases.
Syst Appl Microbiol. 2006 Mar;29(2):120-30. doi: 10.1016/j.syapm.2005.07.011. Epub 2005 Aug 18.
10
Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S-23S rRNA gene intergenic transcribed spacer region.
J Med Microbiol. 2013 Jan;62(Pt 1):133-144. doi: 10.1099/jmm.0.049452-0. Epub 2012 Sep 20.
引用本文的文献
1
The rise and evolving role of subspecies.
Curr Res Microb Sci. 2025 Jun 6;9:100414. doi: 10.1016/j.crmicr.2025.100414. eCollection 2025.
2
: strategies for adapting to aerobic stress.
J Bacteriol. 2025 Jul 24;207(7):e0009025. doi: 10.1128/jb.00090-25. Epub 2025 Jun 6.
3
Reexamining the role of subspecies in clinical and experimental studies.
Gut Microbes. 2024 Jan-Dec;16(1):2415490. doi: 10.1080/19490976.2024.2415490. Epub 2024 Oct 12.
4
surface-displayed FomA () protein generally stimulates protective immune responses in mice.
Front Microbiol. 2023 Sep 20;14:1228857. doi: 10.3389/fmicb.2023.1228857. eCollection 2023.
5
Vertebral osteomyelitis caused by with an associated asymptomatic liver abscess in an immunocompetent adult: A case report and literature review.
IDCases. 2023 Mar 29;32:e01754. doi: 10.1016/j.idcr.2023.e01754. eCollection 2023.
6
Fusobacterium nucleatum and colorectal cancer: From phenomenon to mechanism.
Front Cell Infect Microbiol. 2022 Nov 29;12:1020583. doi: 10.3389/fcimb.2022.1020583. eCollection 2022.
7
Identification of Species Using Matrix-Assisted Laser Desorption/Ionization-Time-of-Flight Mass Spectrometry by Updating ASTA CoreDB.
Yonsei Med J. 2022 Dec;63(12):1138-1143. doi: 10.3349/ymj.2022.0271.
8
Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) Analysis for the Identification of Pathogenic Microorganisms: A Review.
Microorganisms. 2021 Jul 19;9(7):1536. doi: 10.3390/microorganisms9071536.
9
A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer.
Microb Biotechnol. 2021 Sep;14(5):2176-2186. doi: 10.1111/1751-7915.13900. Epub 2021 Jul 26.
10
What Do We See in Spectra?: Assignment of High-Intensity Peaks of and Spectra of MALDI-TOF Mass Spectrometry by Interspecies Comparative Proteogenomics.
Microorganisms. 2021 Jun 8;9(6):1243. doi: 10.3390/microorganisms9061243.
本文引用的文献
1
Fusobacterium nucleatum endocarditis presenting as liver and brain abscesses in an immunocompetent patient.
Am J Med Sci. 2015 Mar;349(3):284-5. doi: 10.1097/MAJ.0000000000000388.
2
Fusobacterium nucleatum: a commensal-turned pathogen.
Curr Opin Microbiol. 2015 Feb;23:141-7. doi: 10.1016/j.mib.2014.11.013. Epub 2015 Jan 8.
3
Mobile microbiome: oral bacteria in extra-oral infections and inflammation.
J Dent Res. 2013 Jun;92(6):485-91. doi: 10.1177/0022034513487559. Epub 2013 Apr 26.
4
Fusobacterium nucleatum subsp. fusiforme Gharbia and Shah 1992 is a later synonym of Fusobacterium nucleatum subsp. vincentii Dzink et al. 1990.
Curr Microbiol. 2013 Apr;66(4):414-7. doi: 10.1007/s00284-012-0289-y. Epub 2012 Dec 24.
5
Maternal oral origin of Fusobacterium nucleatum in adverse pregnancy outcomes as determined using the 16S-23S rRNA gene intergenic transcribed spacer region.
J Med Microbiol. 2013 Jan;62(Pt 1):133-144. doi: 10.1099/jmm.0.049452-0. Epub 2012 Sep 20.
6
Identification of clinical isolates of anaerobic bacteria using matrix-assisted laser desorption ionization-time of flight mass spectrometry.
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2257-62. doi: 10.1007/s10096-012-1563-4. Epub 2012 Feb 28.
7
Fusobacterium nucleatum: an emerging gut pathogen?
Gut Microbes. 2011 Sep 1;2(5):294-8. doi: 10.4161/gmic.2.5.18603.
8
Differentiation of species of the Streptococcus bovis/equinus-complex by MALDI-TOF Mass Spectrometry in comparison to sodA sequence analyses.
Syst Appl Microbiol. 2011 Feb;34(1):52-7. doi: 10.1016/j.syapm.2010.11.010. Epub 2011 Jan 17.
9
Application of rpoB and zinc protease gene for use in molecular discrimination of Fusobacterium nucleatum subspecies.
J Clin Microbiol. 2010 Feb;48(2):545-53. doi: 10.1128/JCM.01631-09. Epub 2009 Dec 2.
10
Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.
Clin Infect Dis. 2009 Aug 15;49(4):543-51. doi: 10.1086/600885.
Zotero 插件