制备和调节阴离子交换层状双氢氧化物微图案阵列的细胞黏附
Preparation and regulating cell adhesion of anion-exchangeable layered double hydroxide micropatterned arrays.
机构信息
State Key Laboratory of Chemical Resource Engineering, §Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, College of Materials Science & Engineering, and ⊥Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology , Beijing 100029, China.
出版信息
ACS Appl Mater Interfaces. 2015 Feb 25;7(7):3882-7. doi: 10.1021/acsami.5b00145. Epub 2015 Feb 9.
We describe a reliable preparation of MgAl-layered double hydroxide (MgAl-LDH) micropatterned arrays on gold substrate by combining SO3(-)-terminated self-assembly monolayer and photolithography. The synthesis route is readily extended to prepare LDH arrays on the SO3(-)-terminated polymer-bonded glass substrate amenable for cell imaging. The anion-exchangeable MgAl-LDH micropattern can act both as bioadhesive region for selective cell adhesion and as nanocarrier for drug molecules to regulate cell behaviors. Quantitative analysis of cell adhesion shows that selective HepG2 cell adhesion and spreading are promoted by the micropatterned MgAl-LDH, and also suppressed by methotrexate drug released from the LDH interlayer galleries.
我们通过结合 SO3(-)端接自组装单层和光刻技术，描述了一种在金基底上制备可靠的 MgAl 层状双氢氧化物 (MgAl-LDH) 微图案阵列的方法。该合成路线易于扩展，可在 SO3(-)端接聚合物键合玻璃基底上制备 LDH 阵列，适用于细胞成像。阴离子可交换的 MgAl-LDH 微图案既可以作为选择性细胞黏附的生物黏附区域，也可以作为药物分子的纳米载体，以调节细胞行为。细胞黏附的定量分析表明，微图案化的 MgAl-LDH 促进了 HepG2 细胞的选择性黏附和铺展，同时也被 LDH 夹层腔室中释放的甲氨蝶呤药物所抑制。
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