用于CpG岛DNA甲基化分析的MIRA测序法。
MIRA-seq for DNA methylation analysis of CpG islands.
作者信息
Jung Marc, Kadam Swati, Xiong Wenying, Rauch Tibor A, Jin Seung-Gi, Pfeifer Gerd P
机构信息
Beckman Research Institute, City of Hope, Duarte, CA 91010, USA.
Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL 60612, USA.
出版信息
Epigenomics. 2015 Aug;7(5):695-706. doi: 10.2217/epi.15.33. Epub 2015 Apr 17.
AIM
To develop a reliable method for whole genome analysis of DNA methylation.
MATERIALS & METHODS: Genome-scale analysis of DNA methylation includes affinity-based approaches such as enrichment using methyl-CpG-binding proteins. One of these methods, the methylated-CpG island recovery assay (MIRA), is based on the high affinity of the MBD2b-MBD3L1 complex for CpG-methylated DNA. Here we provide a detailed description of MIRA and combine it with next generation sequencing platforms (MIRA-seq).
RESULTS
We assessed the performance of MIRA-seq and compared the data with whole genome bisulfite sequencing.
CONCLUSION
MIRA-seq is a reliable, genome-scale DNA methylation analysis platform for scoring DNA methylation differences at CpG-rich genomic regions. The method is not limited by primer or probe design and is cost effective.
目的
开发一种可靠的全基因组DNA甲基化分析方法。
材料与方法
DNA甲基化的基因组规模分析包括基于亲和力的方法，如使用甲基化CpG结合蛋白进行富集。其中一种方法，甲基化CpG岛回收分析（MIRA），基于MBD2b-MBD3L1复合物对CpG甲基化DNA的高亲和力。在此，我们详细描述了MIRA，并将其与新一代测序平台相结合（MIRA-seq）。
结果
我们评估了MIRA-seq的性能，并将数据与全基因组亚硫酸氢盐测序进行了比较。
结论
MIRA-seq是一个可靠的基因组规模DNA甲基化分析平台，用于对富含CpG的基因组区域的DNA甲基化差异进行评分。该方法不受引物或探针设计的限制，且具有成本效益。
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