构建一条杂交途径以提高谷氨酸棒杆菌中莽草酸的产量。
Engineering of a hybrid route to enhance shikimic acid production in Corynebacterium glutamicum.
作者信息
Zhang Bo, Jiang Cheng-Ying, Liu Yi-Ming, Liu Chang, Liu Shuang-Jiang
机构信息
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beichen-Xilu No. 1, Chaoyang District, Beijing, 100101, People's Republic of China.
出版信息
Biotechnol Lett. 2015 Sep;37(9):1861-8. doi: 10.1007/s10529-015-1852-y. Epub 2015 May 13.
OBJECTIVES
To exploit the archaeal shikimic acid (SA) synthesis pathway toenhance SA production in Corynebacterium glutamicum.
RESULTS
Genetic cassettes were constructed that encoded a hybrid route for SA synthesis, which were composed of the archaeal 6-deoxy-5-ketofructose 1-phosphate pathway and the bacterial SA pathway. Corynebacterium glutamicum strains that expressed the hybrid route increased SA production by 57%. A recombinant strain of C. glutamicum that simultaneously overexpressed the hybrid route and its native SA pathway produced 4.7 gSA/l, while C. glutamicum with only the native SA pathway produced 3.7 gSA/l.
CONCLUSIONS
A new hybrid route for SA production was successfully constructed, that effectively increased SA production in C. glutamicum.
目的
利用古菌莽草酸（SA）合成途径提高谷氨酸棒杆菌中SA的产量。
结果
构建了编码SA合成杂交途径的遗传盒，该途径由古菌6-脱氧-5-酮果糖1-磷酸途径和细菌SA途径组成。表达杂交途径的谷氨酸棒杆菌菌株使SA产量提高了57%。同时过表达杂交途径及其天然SA途径的谷氨酸棒杆菌重组菌株产生4.7 gSA/l，而仅具有天然SA途径的谷氨酸棒杆菌产生3.7 gSA/l。
结论
成功构建了一种新的SA生产杂交途径，有效提高了谷氨酸棒杆菌中SA的产量。
Zotero 插件