[AMCase mRNA在SD大鼠变应性鼻炎鼻黏膜中的表达]

Liu Yan, Xing Zhimin, Wang Min

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2015 Feb;29(3):267-9, 274.

OBJECTIVE

To examine AMCase mRNA expression levels in nasal mucosa of allergic rhinitis SD rats.

METHOD

Thirty SD rats were chosen and randomly divided into the experimental group and the control group. 20 in experimental group and 10 in the control group. AR model rats were established through repeated intraperitoneal shot of ovalbumin (OVA) for 2 weeks and consequently confirmed by local challenge with OVA for 1 week. The control group was treated by the same method with Physiological saline water instead of OVA. After the last excitation allergic rhinitis was diagnosed according to the accumulation score about nasal symptom. The septal mucosa of all rats were used to diagnose pathologically by HE dyeing. AMCase mRNA in nasal mucosa, obtained from the bilateral nasal mucosa in two groups, were used to do reverse transcriptive polymerase chain reaction. Real-time polymerase chain reaction was used to examine AMCase mRNA expression levels.

RESULT

(1) The results showed definitely that there were positive expression of AMCase mRNA in normal nasal mucosa. This expression increase significantly during nasal allergy (P < 0.05). (2) The increased expression of AMCase mRNA in allergic rhinitis are related with the nasal symptoms score (r = 0.411, P < 0.05).

CONCLUSION

Increased expression of AMCase mRNA in nasal mucosa in allergic rhinitis model might play roles in the pathogenesis of AR, and Restrain the enzyme activity could become new treatment targets of allergic rhinitis.

目的

检测变应性鼻炎（AR）SD大鼠鼻黏膜中酸性哺乳动物几丁质酶（AMCase）mRNA的表达水平。

方法

选取30只SD大鼠，随机分为实验组和对照组，实验组20只，对照组10只。通过反复腹腔注射卵白蛋白（OVA）建立AR模型大鼠2周，随后用OVA局部激发1周以确诊。对照组用生理盐水代替OVA，采用相同方法处理。末次激发后，根据鼻症状累积评分诊断变应性鼻炎。所有大鼠鼻中隔黏膜均行苏木精-伊红（HE）染色病理诊断。取两组大鼠双侧鼻黏膜，用于逆转录聚合酶链反应检测鼻黏膜中的AMCase mRNA。采用实时聚合酶链反应检测AMCase mRNA表达水平。