凝胶形式强力霉素对骨再生的影响：大鼠颅骨的组织形态计量学和断层扫描研究

Effect of Doxycycline in Gel Form on Bone Regeneration: Histomorphometric and Tomographic Study in Rat Calvaria.

作者信息

Silva Amanda C, Oliveira Marina R, Amaral Lysandro F A, Ferreira Sabrina, Garcia Idelmo R, Mariano Ronaldo C

机构信息

Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, MG, Brazil.

Department of Diagnostics and Surgery, São Paulo Estadual University, Araraquara, SP, Brazil.

出版信息

J Periodontol. 2016 Jan;87(1):74-82. doi: 10.1902/jop.2015.150343. Epub 2015 Aug 20.

DOI:10.1902/jop.2015.150343

PMID:26291295

Abstract

BACKGROUND

This study aims to investigate the effects of the local application of doxycycline in the form of natrosol-based gel on bone regeneration by examining critical defects in rat calvaria.

METHODS

Twenty-four rats were randomly divided into three groups with eight animals each, according to the treatment received: BC (untreated, filled with blood clot), NAT (natrosol gel alone), and DOX (10% doxycycline gel). Four animals from each group were euthanized at 4 and 8 weeks postoperatively. In tomographic analysis, mean density in the region of the defect was calculated as a percentage relative to the native bone density. In histomorphometric analysis, the newly formed bone area was calculated as a percentage of the total area. The values obtained underwent analysis of variance and Tukey testing (P <0.05).

RESULTS

The mean percentage of tomographic density in the region of the defect at the end of 8 weeks was higher for DOX (44.37%), and statistical differences in this period were observed between DOX and NAT (33.10%) and DOX and BC (32.43%). Regarding new bone formation, at the end of 8 weeks, DOX (61.11%) also had the highest mean bone formation, and statistical differences were observed between DOX and NAT (34.61%) and DOX and BC (23.11%).

CONCLUSION

It was observed that 10% doxycycline gel had a good effect on bone regeneration regarding the filling of critical defects in rat calvaria.

摘要

背景

本研究旨在通过检查大鼠颅骨的关键缺损，研究以羟丙基甲基纤维素凝胶形式局部应用强力霉素对骨再生的影响。

方法

24只大鼠根据接受的治疗随机分为三组，每组8只动物：BC组（未治疗，填充血凝块）、NAT组（仅用羟丙基甲基纤维素凝胶）和DOX组（10%强力霉素凝胶）。每组4只动物在术后4周和8周实施安乐死。在断层扫描分析中，缺损区域的平均密度以相对于正常骨密度的百分比计算。在组织形态计量分析中，新形成的骨面积以总面积的百分比计算。对获得的值进行方差分析和Tukey检验（P<0.05）。

结果

8周结束时，DOX组缺损区域的断层扫描密度平均百分比更高（44.37%），在此期间，DOX组与NAT组（33.10%）以及DOX组与BC组（32.43%）之间观察到统计学差异。关于新骨形成，在8周结束时，DOX组（61.11%）的平均骨形成也最高，DOX组与NAT组（34.61%）以及DOX组与BC组（23.11%）之间观察到统计学差异。