基于真菌细胞壁聚合物的纳米粒子在保护番茄植株免受尖孢镰刀菌番茄专化型引起的萎蔫病中的作用。
Fungal cell wall polymer based nanoparticles in protection of tomato plants from wilt disease caused by Fusarium oxysporum f.sp. lycopersici.
机构信息
Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
出版信息
Carbohydr Polym. 2015 Nov 20;133:400-7. doi: 10.1016/j.carbpol.2015.07.066. Epub 2015 Jul 26.
Cell wall polymer (chitosan) was isolated from Fusarium oxysporum f.sp. lycopersici. They were cross linked with sodium tripolyphosphate (TPP) to synthesize nanoparticles (CWP-NP). The nanoparticles were characterized by FTIR, DLS, SEM, XRD and NMR analyses. The isolated CWP-NP exhibit antifungal activity under in vitro condition. The foliar application of the CWP-NP to tomato plants challenged with F. oxysporum f. sp. lycopersici showed delay in wilt disease symptom expression and reduce the wilt disease severity. Treated plants also showed enhanced yield. These results suggested the role of the CWP-NP in protecting tomato plants from F. oxysporum f.sp. lycopersici infection.
从尖孢镰刀菌茄腐专化型（Fusarium oxysporum f.sp. lycopersici）中分离出细胞壁聚合物（壳聚糖）。它们与三聚磷酸钠（TPP）交联，合成纳米颗粒（CWP-NP）。通过傅里叶变换红外光谱（FTIR）、动态光散射（DLS）、扫描电子显微镜（SEM）、X 射线衍射（XRD）和核磁共振（NMR）分析对纳米颗粒进行了表征。分离的 CWP-NP 在体外条件下表现出抗真菌活性。将 CWP-NP 叶面施用于番茄植株，番茄植株受到尖孢镰刀菌茄腐专化型的挑战，表现出发病症状延迟和枯萎病严重程度降低。处理过的植物也表现出产量增加。这些结果表明 CWP-NP 在保护番茄植株免受尖孢镰刀菌茄腐专化型感染方面发挥作用。
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