一个含 Kelch 结构域的 F-Box 编码基因负调控甜瓜中黄酮类化合物的积累。
A Kelch Domain-Containing F-Box Coding Gene Negatively Regulates Flavonoid Accumulation in Muskmelon.
作者信息
Feder Ari, Burger Joseph, Gao Shan, Lewinsohn Efraim, Katzir Nurit, Schaffer Arthur A, Meir Ayala, Davidovich-Rikanati Rachel, Portnoy Vitaly, Gal-On Amit, Fei Zhangjun, Kashi Yechezkel, Tadmor Yaakov
机构信息
Cucurbit Section, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay 3009500, Israel (A.F., J.B., E.L., N.K., A.M., R.D.-R., V.P., Y.T.);Faculty of Biotechnology and Food Engineering, Technion, Israel Institute of Technology, Haifa 32000, Israel (A.F., Y.K.);Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853 (S.G., Z.F.); andPlant Science (A.A.S.) andPlant Protection (A.G.) Institutes, ARO, The Volcani Center, Bet Dagan 50250, Israel.
Cucurbit Section, Newe Ya'ar Research Center, Agricultural Research Organization (ARO), Ramat Yishay 3009500, Israel (A.F., J.B., E.L., N.K., A.M., R.D.-R., V.P., Y.T.);Faculty of Biotechnology and Food Engineering, Technion, Israel Institute of Technology, Haifa 32000, Israel (A.F., Y.K.);Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853 (S.G., Z.F.); andPlant Science (A.A.S.) andPlant Protection (A.G.) Institutes, ARO, The Volcani Center, Bet Dagan 50250, Israel
出版信息
Plant Physiol. 2015 Nov;169(3):1714-26. doi: 10.1104/pp.15.01008. Epub 2015 Sep 10.
The flavonoids are phenylpropanoid-derived metabolites that are ubiquitous in plants, playing many roles in growth and development. Recently, we observed that fruit rinds of yellow casaba muskmelons (Cucumis melo 'Inodorous Group') accumulate naringenin chalcone, a yellow flavonoid pigment. With RNA-sequencing analysis of bulked segregants representing the tails of a population segregating for naringenin chalcone accumulation followed by fine mapping and genetic transformation, we identified a Kelch domain-containing F-box protein coding (CmKFB) gene that, when expressed, negatively regulates naringenin chalcone accumulation. Additional metabolite analysis indicated that downstream flavonoids are accumulated together with naringenin chalcone, whereas CmKFB expression diverts the biochemical flux toward coumarins and general phenylpropanoids. These results show that CmKFB functions as a posttranscriptional regulator that diverts flavonoid metabolic flux.
类黄酮是苯丙烷类衍生的代谢产物，在植物中普遍存在，在生长和发育中发挥着多种作用。最近，我们观察到黄皮甜瓜（黄瓜 '无香组'）的果皮积累柚皮素查耳酮，一种黄色类黄酮色素。通过对代表柚皮素查耳酮积累分离群体尾部的混合分离群体进行RNA测序分析，随后进行精细定位和遗传转化，我们鉴定出一个含有Kelch结构域的F-box蛋白编码（CmKFB）基因，该基因表达时会负向调节柚皮素查耳酮的积累。进一步的代谢物分析表明，下游类黄酮与柚皮素查耳酮一起积累，而CmKFB的表达会使生化通量转向香豆素和一般苯丙烷类化合物。这些结果表明，CmKFB作为一种转录后调节因子，可改变类黄酮代谢通量。
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