Dong Lee Soon
Faculty of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
Int J Syst Evol Microbiol. 2016 Feb;66(2):598-603. doi: 10.1099/ijsem.0.000762. Epub 2015 Nov 10.
A Gram-reaction-variable, strictly aerobic, motile, rod-shaped bacterium, designated strain C4-5, was isolated from soil of a natural cave. Cells were oxidase- and catalase-positive and formed endospores in sporangia. The 16S rRNA gene sequence comparison showed that the organism formed a distinct clade within the genus and was most closely related to CKOBP-6 ( = KCTC 13623) (96.85 % 16S rRNA gene sequence similarity) followed by CAU 1005 ( = KCTC 33036) (94.82 %). The following chemotaxonomic features of strain C4-5 are typical for the genus : -diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan, MK-7 as the predominant menaquinone, the major fatty acids of anteiso-C, iso-C and C and the DNA G+C content of 54.8 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and four unidentified aminophospholipids. The phenotypic and phylogenetic data presented support that strain C4-5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is C4-5 ( = KCTC 33652 = DSM 100100).
从一个天然洞穴的土壤中分离出一株革兰氏反应可变、严格需氧、运动、杆状的细菌,命名为C4-5菌株。细胞氧化酶和过氧化氢酶呈阳性,在孢子囊中形成内生孢子。16S rRNA基因序列比较表明,该菌株在该属内形成了一个独特的分支,与CKOBP-6(=KCTC 13623)关系最为密切(16S rRNA基因序列相似性为96.85%),其次是CAU 1005(=KCTC 33036)(94.82%)。C4-5菌株的以下化学分类特征是该属的典型特征:-二氨基庚二酸作为细胞壁肽聚糖中的诊断性二氨基酸,MK-7作为主要的甲基萘醌,anteiso-C、iso-C和C的主要脂肪酸,以及DNA G+C含量为54.8mol%。主要极性脂类为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和四种未鉴定的氨基磷脂。所提供的表型和系统发育数据支持C4-5菌株代表该属的一个新物种,为此提出新种名为sp. nov.。模式菌株为C4-5(=KCTC 33652=DSM 100100)。