炭疽芽孢杆菌17 JB菌株的基因特征分析
Genetic Characterization of Bacillus anthracis 17 JB strain.
作者信息
Seyed-Mohamadi Sakineh, Moradi Bidhendi Soheila, Tadayon Keyvan, Ghaderi Rainak
机构信息
Department of Aerobic Veterinary Bacteria, Razi Institute, Karaj, Iran.
Department of Microbiology, Razi Institute, Karaj, Iran.
出版信息
Iran J Microbiol. 2015 Jun;7(3):168-72.
BACKGROUND AND OBJECTIVES
Bacillus anthracis is one of the most homogenous bacteria ever described. Some level of diversity. Bacillus anthracis 17JB is a laboratory strain It is broadly used as a challenge strain in guinea pigs for potency test of anthrax vaccine.
MATERIAL AND METHODS
This work describes genetic characterization of B. anthracis 17 JB strain using the SNPs and MLVA genotyping.
RESULTS AND CONCLUSION
In SNPs typing, the originally French 17JB strain represented the A.Br. 008/009 subgroup. In Levy's genotyping method, 843, 451 and 864 bp long fragments were identified at AA03, AJ03 and AA07 loci, respectively. In the vaccine manufacturer perspective these findings are much valuable on their own account, but similar research is required to extend molecular knowledge of B. anthracis epidemiology in Persia.
背景与目的
炭疽芽孢杆菌是有史以来描述的最同质化的细菌之一。存在一定程度的多样性。炭疽芽孢杆菌17JB是一株实验室菌株，广泛用作豚鼠的攻击菌株以进行炭疽疫苗的效力测试。
材料与方法
本研究利用单核苷酸多态性（SNPs）和多位点可变数目串联重复序列分析（MLVA）基因分型描述炭疽芽孢杆菌17JB菌株的基因特征。
结果与结论
在SNPs分型中，最初源自法国的17JB菌株属于A.Br.008/009亚群。在Levy基因分型方法中，分别在AA03、AJ03和AA07位点鉴定出长度为843、451和864 bp的片段。从疫苗生产商的角度来看，这些发现本身就很有价值，但还需要进行类似研究以扩展对波斯地区炭疽芽孢杆菌流行病学的分子认识。
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