细胞运动的反幂律行为通过光学相干断层扫描波动光谱揭示了三维共培养中基质-上皮细胞的相互作用。
Inverse-power-law behavior of cellular motility reveals stromal-epithelial cell interactions in 3D co-culture by OCT fluctuation spectroscopy.
作者信息
Oldenburg Amy L, Yu Xiao, Gilliss Thomas, Alabi Oluwafemi, Taylor Russell M, Troester Melissa A
机构信息
Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3255; Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7513; Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295.
Department of Physics and Astronomy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-3255; Biomedical Research Imaging Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7513.
出版信息
Optica. 2015 Oct 20;2(10):877-885. doi: 10.1364/OPTICA.2.000877. Epub 2015 Oct 9.
The progression of breast cancer is known to be affected by stromal cells within the local microenvironment. Here we study the effect of stromal fibroblasts on the in-place motions (motility) of mammary epithelial cells within organoids in 3D co-culture, inferred from the speckle fluctuation spectrum using optical coherence tomography (OCT). In contrast to Brownian motion, mammary cell motions exhibit an inverse power-law fluctuation spectrum. We introduce two complementary metrics for quantifying fluctuation spectra: the power-law exponent and a novel definition of the motility amplitude, both of which are signal- and position-independent. We find that the power-law exponent and motility amplitude are positively (<0.001) and negatively (<0.01) correlated with the density of stromal cells in 3D co-culture, respectively. We also show how the hyperspectral data can be visualized using these metrics to observe heterogeneity within organoids. This constitutes a simple and powerful tool for detecting and imaging cellular functional changes with OCT.
已知乳腺癌的进展会受到局部微环境中基质细胞的影响。在此，我们研究了基质成纤维细胞对三维共培养类器官内乳腺上皮细胞原位运动（迁移能力）的影响，该影响是通过使用光学相干断层扫描（OCT）从散斑波动光谱推断得出的。与布朗运动不同，乳腺细胞运动呈现出反幂律波动光谱。我们引入了两种互补的度量来量化波动光谱：幂律指数和迁移能力幅度的新定义，这两者均与信号和位置无关。我们发现，在三维共培养中，幂律指数与基质细胞密度呈正相关（<0.001），而迁移能力幅度与基质细胞密度呈负相关（<0.01）。我们还展示了如何使用这些度量来可视化高光谱数据，以观察类器官内的异质性。这构成了一种利用OCT检测和成像细胞功能变化的简单而强大的工具。
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