斯里兰卡变异传染性支气管炎病毒的检测（2012 - 2015年）

Detection of variant infectious bronchitis viruses in Sri Lanka (2012-2015).

作者信息

Ball Christopher, Forrester Anne, Ganapathy Kannan

机构信息

Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire, CH64 7TE, UK.

出版信息

Arch Virol. 2016 Jun;161(6):1697-9. doi: 10.1007/s00705-016-2831-x. Epub 2016 Mar 28.

DOI:10.1007/s00705-016-2831-x

PMID:27020570

Abstract

Poultry production is an important sector of agriculture in Sri Lanka; however, there is a lack of information regarding circulation of infectious bronchitis virus (IBV). RNA was extracted from chicken tissues, subjected to IBV S1 RT-PCR, and sequenced. Overall, 19 out of 34 (55.88 %) samples were IBV positive and contained the genotype 793B (n = 13; 68.42 %), D274 (n = 4; 21.05 %) or Massachusetts (n = 2; 10.53 %). All three genotypes contained at least one strain with less than 99 % nucleotide sequence identity to the corresponding vaccine strains. This report identified co-circulation of IBV strains 793B, Massachusetts and D274, in Sri Lanka that are divergent from the respective vaccine strains.

摘要

家禽养殖是斯里兰卡农业的一个重要部门；然而，关于传染性支气管炎病毒（IBV）传播的信息却很匮乏。从鸡组织中提取RNA，进行IBV S1 RT-PCR检测并测序。总体而言，34个样本中有19个（55.88%）呈IBV阳性，包含793B基因型（n = 13；68.42%）、D274基因型（n = 4；21.05%）或马萨诸塞基因型（n = 2；10.53%）。所有这三种基因型都至少包含一个与相应疫苗株核苷酸序列同一性低于99%的毒株。本报告确定了在斯里兰卡，IBV毒株793B、马萨诸塞和D274共同传播，且与各自的疫苗株存在差异。