质谱流式细胞术（CyTOF）在自然杀伤细胞功能和表型分析中的应用。
Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells.
作者信息
Kay Alexander W, Strauss-Albee Dara M, Blish Catherine A
机构信息
Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Stanford Immunology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
出版信息
Methods Mol Biol. 2016;1441:13-26. doi: 10.1007/978-1-4939-3684-7_2.
Abstract
Mass cytometry is a novel platform for high-dimensional phenotypic and functional analysis of single cells. This system uses elemental metal isotopes conjugated to monoclonal antibodies to evaluate up to 42 parameters simultaneously on individual cells with minimal overlap between channels. The platform can be customized for analysis of both phenotypic and functional markers. Here, we will describe methods to stain, collect, and analyze intracellular functional markers and surface phenotypic markers on natural killer cells.
摘要
质谱流式细胞术是用于单细胞高维表型和功能分析的新型平台。该系统使用与单克隆抗体偶联的元素金属同位素，以在各通道间最小程度重叠的情况下，对单个细胞同时评估多达42个参数。该平台可定制用于表型和功能标志物的分析。在此，我们将描述对自然杀伤细胞上的细胞内功能标志物和表面表型标志物进行染色、收集及分析的方法。
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