人参皂苷Rh2通过肿瘤坏死因子-α信号通路抑制人白血病细胞的增殖并诱导其凋亡。
Ginsenoside Rh2 inhibits proliferation and induces apoptosis in human leukemia cells via TNF-α signaling pathway.
作者信息
Huang Jingjia, Peng Kunjian, Wang Linghao, Wen Bin, Zhou Lin, Luo Tiao, Su Min, Li Jijia, Luo Zhiyong
机构信息
Molecular Biology Research Center, State Key Laboratory of Medical Genetics, School of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 410008, China.
Molecular Biology Research Center, State Key Laboratory of Medical Genetics, School of Life Sciences, Xiangya School of Medicine, Central South University, Changsha 410008, China
出版信息
Acta Biochim Biophys Sin (Shanghai). 2016 Aug;48(8):750-5. doi: 10.1093/abbs/gmw049. Epub 2016 May 13.
Ginsenoside Rh2, a triterpene saponin extracted from Panax ginseng, exhibits pharmacological activity against multiple cancers. However, the anticancer mechanism of ginsenoside Rh2 is unclear. In this study, we found that ginsenoside Rh2 effectively inhibits growth and induces apoptosis of HL-60 cells. Using microarray technology, we found that tumor necrosis factor-α (TNF-α) is clearly up-regulated. Furthermore, anti-TNF-α antibody relieved the Rh2-induced HL-60 cell apoptosis via suppression of caspase-8, caspase-9, and caspase-3 activation. In addition, TNF-α up-regulation was also observed in other Rh2-treated cancer cell lines. These results demonstrate that TNF-α plays a key role in ginsenoside Rh2-induced cell apoptosis.
人参皂苷Rh2是从人参中提取的一种三萜皂苷，对多种癌症具有药理活性。然而，人参皂苷Rh2的抗癌机制尚不清楚。在本研究中，我们发现人参皂苷Rh2能有效抑制HL-60细胞的生长并诱导其凋亡。利用微阵列技术，我们发现肿瘤坏死因子-α（TNF-α）明显上调。此外，抗TNF-α抗体通过抑制caspase-8、caspase-9和caspase-3的激活，减轻了Rh2诱导的HL-60细胞凋亡。此外，在其他经Rh2处理的癌细胞系中也观察到TNF-α上调。这些结果表明，TNF-α在人参皂苷Rh2诱导的细胞凋亡中起关键作用。
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